| 摘要: |
| [摘要] 目的 通过小分子干扰RNA(siRNA)沉默Livin的表达，探讨对胶质瘤U251细胞的增殖、凋亡的影响。方法 制备Livin siRNA重组腺病毒，感染U251细胞,采用RT-PCR、Western blot检测Livin基因的干扰效果,用MTT和流式细胞仪检测其U251细胞的增殖、凋亡。同时在体内观察重组腺病毒对荷瘤小鼠的治疗效应。结果 重组腺病毒Ad-Livin siRNA能有效抑制U251细胞的Livin mRNA和蛋白水平的表达；抑制U251细胞的增殖,促进凋亡；在体内有效抑制U251细胞的生长和提高荷瘤小鼠的平均生存期。结论 用重组腺病毒介导抑制Livin基因的表达,可抑制U251细胞的增殖,促进凋亡，为胶质瘤的治疗提供了新的策略。 |
| 关键词: 重组腺病毒 RNA干扰 Livin U251 |
| DOI：10.3724/SP.J.1008.2011.0603 |
| 投稿时间：2010-12-11修订日期：2011-04-01 |
| 基金项目: |
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| The effect study of recombinant adenovirus Livin siRNA on the proliferation and apoptosis of U251 cells |
| 陈宏颉 |
| (Fuzhou General Hospital of Nanjing Command) |
| Abstract: |
| [Abstract] Objective To investigate the expression and effect of Livin gene silencing by siRNA on growth and apoptosis in U251 cells. Methods The targeting Livin siRNA was prepared and transfected into U251 cells. The gene silencing efficiency was monitored by RT-PCR and Western blot analysis. The proliferation and apoptosis of U251 cells were detected by MTT assays and flow cytometry. The tumor volume and mean life span of tumor bearing mice were also observed. Results Recombinant adenovirus could silence Livin gene at the level of mRNA and protein, inhibited proliferation and enhanced apoptosis of U251 cells. In addition, Recombinant adenovirus could also inhibit the tumor growth and prolong the lifespan of tumor bearing mice. Conclusion The recombinant adenovirus Livin siRNA effectively silenced the expression of Livin gene, inhibited proliferation and enhanced apoptosis which provided a promising therapeutical strategy for glioma. |
| Key words: recombinant adenovirus RNA interference Livin U251 |
