| 摘要: |
| 目的合成一种新型阳离子聚合物聚氨基酯(PBAE),并以此作为基因载体进行研究。方法通过迈克尔加成反应合成PBAE，以自主装法制备PBAE/pDNA纳米复合物，并考察PBAE/pDNA纳米复合物在HEK293细胞中的转染效率。结果当PBAE/pDNA的质量比为501时，pDNA被完全包裹；在HEK293细胞转染实验中,当PBAE/pDNA质量比为2001时，其转染效率高于阳性对照组PEI/pDNA的转染效率(43.3%±3.7% vs 30.3%±2.1%, P<0.05)。结论带正电荷的PBAE能够通过静电作用浓缩包裹pDNA，在体外转染实验中具有较高的转染效率。 |
| 关键词: 聚氨基酯 纳米粒 转染效率 基因载体 |
| DOI：10.3724/SP.J.1008.2011.0473 |
| 投稿时间：2011-01-04修订日期：2011-03-23 |
| 基金项目:上海市科委纳米专项基金(0852nm04200). |
|
| Polymers poly(β-amino esters) nanoparticles for gene delivery |
| WANG Jiang-feng,LU Ying,HUANG Jing-bin,ZOU Hao,ZHANG He,ZHONG Yan-qiang* |
| (Department of Pharmaceutics, School of Pharmacy, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| ObjectiveTo synthesize a new type of cationic polymers (β-amino esters)(PBAE) and to study its role as a gene vector. MethodsPBAE was synthesized by the Michael addition reaction. PBAE/pDNA complex nanoparticles were prepared by self-assembly, and the gene tranfection efficiency mediated by the nanoparticles into HEK293 cells in vitro was observed. ResultsWhen weight ratio of PBAE/pDNA was 501, pDNA was completely wrapped. And the transfection efficiency of PBAE/pDNA nanoparticles (PBAE/pDNA weight ratio being 2001) was significantly higher than PEI/pDNA nanoparticles(43.3%±3.7% vs 30.3%±2.1%, P<0.05). ConclusionCationic poly(β-amino esters) can electrostatically bind to and condense anionic DNA into nanometersized particles. PBAE/pDNA nanoparticles has a high transfection efficiency in in vitro transfection experiments. |
| Key words: polymers poly-amino ester nanoparticles transfection efficiency gene vectors |
