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| 重组人促红细胞生成素对大鼠脑缺血再灌注损伤后血脑屏障内皮屏障抗原及通透性的影响 |
| 刘式威1*,刘政华2,吕霞2,杨桂蓉3,刘志刚1,尹岭4 |
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| (1. 解放军211医院神经内科,哈尔滨 150080;2. 解放军65426部队医院,哈尔滨 150086;7;3. 哈尔滨香坊区民生社区卫生服务中心,哈尔滨 150040;4. 解放军总医院神经信息中心,北京 100837) |
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| 摘要: |
| 【摘要】 目的 观察促红细胞生成素(EPO)对大鼠脑缺血再灌注损伤后血脑屏障EBA及通透性的影响。方法 20只雄性SD大鼠随机分成EPO治疗组、缺血再灌注组，采用阻断大鼠一侧大脑中动脉(MCA)血流2小时，再灌注24小时制成局灶性脑缺血模型。于再灌注前，EPO治疗组经腹腔注入EPO(3000U/Kg)；缺血再灌注组给予等量生理盐水腹腔注射，24小时后断头取脑，制作冰冻切片，免疫组化染色检测血脑屏障EBA和fibrinogen的表达。结果 缺血再灌注组EBA表达数5.30±1.24，EPO治疗组EBA表达数10.60±0.93，，EPO治疗组较缺血再灌注组显著增加(P<0.01)。缺血再灌注组Fibrinogen表达数8.60±3.12，EPO治疗组fibrinogen表达数5.50±2.56，，EPO治疗组较缺血再灌注组显著减少(P<0.01)。结论 EPO能显著增加脑缺血再灌注损伤后血脑屏障EBA表达，减少fibrinogen外渗，通过提高EBA含量，降低血脑屏障通透性，从而发挥对血脑屏障的保护作用。 |
| 关键词: 重组人促红细胞生成素 脑缺血 再灌注损伤；血脑屏障 |
| DOI：10.3724/SP.J.1008.2011.0802 |
| 投稿时间：2011-02-13修订日期：2011-02-15 |
| 基金项目:国家重点基础研究发展计划（“973”计划,2004CB720302）. |
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| Effects of rhEPO on endothelial barrier antigen expression and blood-brain barrier permeability after brain ischemia reperfusion injury in rats |
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| Abstract: |
| 【Abstract】 Objective To investigate the effects of EPO on EBA expression and BBB permeability after ischemia reperfusion injury in rat.Methods 20 male SD rats were randomly divided into EPO treatment group、ischemia reperfusion group .The model of focal cerebral ischemia reperfusion injury was established by occluding middle cerebral artery(MCA) for 2h and reperfusing for 24h.EPO treatment group received rHu-EPO at 3000U/Kg of bodyweight,while ischemia reperfusion group received the same dose of normal saline,just before the beginning of reperfusion. The rat prefrontal cortex was removed 24h after reperfusion. the expression of EBA and fibrinogen were observed using immunohistology.Results By IHC method,the number of EBA positive microvessels was 10.60±0.93 in EPO treatment group,5.30±1.24 in ischemia reperfusion group. the number of EBA was significantly increased in EPO treatment group compared with those in ischemia reperfusion group(P<0.01);The expression of fibrinogen was 5.50±2.56 in EPO treatment group,8.60±3.12 in ischemia reperfusion group. the number of fibrinogen was significantly decreased in EPO treatment group compared with those in ischemia reperfusion group (P<0.01).Conclusions EPO treatment could increase EBA expression and reduce fibrinogen expression after ischemia reperfusion injury.EPO may exert BBB protective effect through enhancing the content of EBA ,inhibiting BBB permeability. |
| Key words: 【Key words】Erythropoietin Blood brain barrier Ischemia reperfusion injury Endothelial barrier antigen |
