| 摘要: |
| 目的建立优化的HPLC方法同时测定不同产地罗布麻药材中芦丁、金丝桃苷、异槲皮苷以及槲皮素含量。方法采用Shiseido C18(3.0 μm,3.0 mm×100 mm)色谱柱；以乙腈甲醇=101作为A相-0.1%甲酸水溶液作为B相,梯度洗脱(0~4 min,5%→16%A；4~15 min,16%→23%A；15~20 min,23%→35%A；20~22 min,35%→55%A),流速 0.6 ml·min-1；二极管阵列检测器检测波长:360 nm；柱温:30℃；进样量:5 μl。结果芦丁、金 |
| 关键词: 高压液相色谱法 罗布麻 芦丁 金丝桃苷 异槲皮苷 槲皮素 |
| DOI：10.3724/SP.J.1008.2011.0759 |
| 投稿时间：2011-02-22修订日期：2011-06-23 |
| 基金项目: |
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| Optimized HPLC-DAD in simultaneous determination of 4 flavonoids in Apocynum venetum |
| LIANG Shan-shan,ZHAO Liang,ZHANG Hai,JIA Jing,LI Yue-yue,ZHANG Guo-qing* |
| (Department of Pharmacy, Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai 200438, China) |
| Abstract: |
| Objective：To develop an HPLC method for simultaneous determination of Rutin, Hyperoside, Isoquercitrin, Quercetin in the hebal of Apocynum venetum from different places．Methods：The separation was performed on a Shiseido C18(3μm，30㎜×100㎜) column by gradient elution with acetonitrile：methanol=10:1 as the mobile phase A 一0．1％ formic acid as the mobile phase B （0-4min，5%A→16%A；4-15min，16%→23%A；15-20min，23%A→35%A;20-22min，35%A→55%A）at a flow rate of 0.6 mL•min-1．The detection wave length of DAD was set at 360 nm，and the column temperature was 30 ℃．The injection volumn was 5μl。Results： Four flavonoids(Rutin, Hyperoside, Isoquercitrin, Quercetin) were separated at base line within 22 min with good linearity (r=0．9999) .The result of intra-day and inter-day precisions ,limits of detection and quantitation were all within the normal range. The recovery rates (n=3)were Rutin 102.0%，96.40%，103.8%；Hyperoside 98.50%，101.0%，102.9%；Isoquercitrin 98.40%，100.4%，101.4%；Quercetin 104.4%，103.1%，103.5%。respectively．The contents of four flavonoids in the hebal of Apocynum venetum from 9 different places were determined.Conclusion：The developed method is rapid，simple，accurate，reliable，and is helpful to control the quality of Apocynum venetum． |
| Key words: HPLC Apocynum venetum. Rutin Hyperoside, Isoquercitrin Quercetin |
