| 摘要: |
| 目的研究穿心莲内酯对细胞因子肿瘤坏死因子-α和白介素-12水平的影响。方法用巯基乙酸腹腔注射小鼠,取小鼠的腹腔巨噬细胞进行体外培养,以穿心莲内酯预先作用于巨噬细胞,随后添加脂多糖刺激巨噬细胞,以RT-PCR检测巨噬细胞肿瘤坏死因子-α、白介素-12a和白介素-12b mRNA水平,以ELISA检测巨噬细胞培养上清的肿瘤坏死因子-α和白介素-12蛋白水平。结果穿心莲内酯能抑制活化小鼠腹腔巨噬细胞的肿瘤坏死因子-α mRNA和蛋白水平,并能抑制白介素-12a mRNA、白介素-12b mRNA和白介素-12蛋 |
| 关键词: 穿心莲；穿心莲内酯；肿瘤坏死因子α 白介素12 |
| DOI：10.3724/SP.J.1008.2011.0717 |
| 投稿时间：2011-02-23修订日期：2011-06-29 |
| 基金项目:国家自然科学基金 |
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| Andrographolide inhibits expression of TNF-α and IL-12 in activated macrophages |
| QIN Lin-hua1*,ZHENG Zhi-wu1,KONG Ling2,L Jiao1,SHI Yun-xing1,LI Yong-ping1 |
| (1. Department of Gastroenterology, No. 411 Hospital of PLA, Shanghai 200081, China;2. Shanghai Health Center, Chinese Academy of Sciences, Shanghai 200025, China) |
| Abstract: |
| [ABSTRACT] Objective: To observe the effect of andrographolide on the expression of tumor necrosis factor -alpha and interleukin-12 in activated macrophages. Methods: Mouse peritoneal macrophages were harvested from mice that had been injected i.p. earlier with thioglycollate,were then cultured in media with andrographolide, lipopolysaccharide was then added to the media.Tumor necrosis factor -alpha , interleukin-12a and interleukin-12b mRNA of the macrophages were detected by RT-PCR. Tumor necrosis factor alpha and interleukin-12 protein in the supernatants were detected by ELISA. Results：Andrographolide inhibit tumor necrosis factor -alpha , interleukin-12a and interleukin-12b mRNA as detected by RT-PCR in activated macrophages. Andrographolide inhibit both tumor necrosis factor alpha and interleukin-12 protein in a concentration-dependent manner as detected by ELISA. 1ug/ml andrographolide partially suppressed the production of tumor necrosis factor alpha, and 1ug/ml andrographolide significantly inhibited tumor necrosis factor alpha production. 1ug/ml andrographolide significantly inhibited nterleukin-12 production.. Conclusion: Andrographolide can inhibit the expression of both tumor necrosis factor and interleukin-12. |
| Key words: Andrographis paniculata Andrographolide cytokines |
