| 摘要: |
| 目的观察miRNA-29c(miR-29c)对前列腺癌细胞系LNCaP增殖和侵袭能力的影响,探讨其潜在的应用价值。方法采用qRT-PCR观察雄激素依赖性(LNCaP)与雄激素非依赖性(LNCaP-AI)前列腺癌细胞中miR-29c的表达差异；采用miR-29c抑制物(anti-miR-29c)下调LNCaP细胞中miR-29c的表达量,绘制LNCaP细胞生长曲线,利用流式细胞仪测定细胞周期；Transwell侵袭实验分析miR-29c抑制前后LNCaP细胞的体外侵袭能力。结果qRT-PCR显示LNCaP-AI细胞中miR-29c水平明显低于LNCaP细胞(P<0.05)；下调LNCaP细胞中miR-29c的表达量,可明显促进LNCaP细胞体外增殖及侵袭能力(P<0.05)。结论miR-29c的正常表达有利于抑制LNCaP细胞的体外增殖和侵袭,有望成为前列腺癌生物治疗的潜在靶点。 |
| 关键词: 前列腺肿瘤 miR-29c 细胞增殖 肿瘤浸润 |
| DOI：10.3724/SP.J.1008.2011.0607 |
| 投稿时间：2011-03-08修订日期：2011-03-21 |
| 基金项目: |
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| Inhibitory effect of microRNA-29c on proliferation and invasion of human prostate cancer cell line LNCaP |
| DENG Zhen△,TANG Liang△,JIAO Li,XU Chuan-liang,ZHANG Zhen-sheng,ZENG Qin-song,SUN Ying-hao* |
| (Department of Urology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| ObjectiveTo observe the effect of microRNA-29c on the proliferation and invasion abilities of human prostate cancer cell line LNCaP, and to discuss its potential application. MethodsThe difference in miR-29c expression between ADPC (androgen-dependent prostate cancer) and AIPC (androgen-independent prostate cancer) cell lines was observed by real time RT-PCR．MiR-29c-inhibitor(anti-miR-29c)was used to decrease miR-29c expression in LNCaP cells; then the cell proliferation was examined with CCK-8 assay, the cell cycle was analyzed using flow cytometry, and the invasive abilities of LNCaP cells were observed by transwell invasion assay before and after treatment with anti-miR-29c. ResultsThe result of real-time RT-PCR showed that miR-29c expression in LNCaP-AI cells was significantly lower than that in LNCaP cells (P<0.05). Down-expression of miR-29c in LNCaP cells significantly promoted the proliferation and invasion abilities of LNCaP cells (P<0.05). ConclusionNormal expression of miR-29c plays an inhibitory role in the proliferation and invasion of LNCaP cells, indicating it might be a new target for biotherapy of prostate cancer. |
| Key words: prostatic neoplasms miR-29c cell proliferation neoplasm invasiveness |
