| 摘要: |
| 目的探讨骨关节炎中基质金属蛋白酶9(MMP-9)、白介素6 (IL-6) mRNA和蛋白的表达。方法采用免疫组织化学技术检测60例骨关节炎病例(OA组)及20例正常对照关节软骨及滑膜MMP-9、IL-6蛋白的表达水平；采用原位分子杂交技术检测30例OA及10例正常对照关节软骨及滑膜MMP-9、IL-6 mRNA的表达水平。结果MMP-9 mRNA和蛋白在OA组阳性表达的积分光密度(IOD)值均高于正常对照组\[(8.38±2.29) vs (3.52±1.36)，P<0.01；(9.18±3.58) vs (3.83±1.48)，P<0.01\]，OA中二者的表达正相关性(r=0.924，P<0.01)；IL-6 mRNA和蛋白在OA组阳性表达的IOD值均高于正常对照组\[(8.74±3.62) vs (5.91±1.53)，P<0.01；(8.96±3.57) vs (6.87±1.43)，P<0.01\]，OA中二者的表达亦正相关(r=0.917，P<0.01)；MMP-9 mRNA、IL-6 mRNA及相应蛋白在OA中的表达均正相关(原位杂交r=0.426，P<0.05；免疫组化r=0.444，P<0.01)。结论MMP-9、IL-6在OA关节软骨与滑膜中呈高表达，二者对OA的发生发展可能起促进作用。 |
| 关键词: 骨关节炎 基质金属蛋白酶9 白介素6 原位杂交 免疫组织化学 |
| DOI：10.3724/SP.J.1008.2011.0672 |
| 投稿时间：2011-03-02修订日期：2011-04-21 |
| 基金项目:河北省人事厅留学归国人员科技活动基金(200911). |
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| Expression of metalloproteinase-9 and interleukin-6 in articular cartilage and synovium of osteoarthritis patients |
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| Abstract: |
| [Abstract] Objective To investigate the significance of expression of MMP-9, IL-6 mRNA and corresponding proteins in Osteoarthritis. Methods Immunohistochemical(IHC) analysis was used to determine the distribution of MMP-9 and IL-6 protein in chondrocytes and synoviocytes of osteoarthritis from 60 patients with osteoarthritis group and 20 patients with normal control group. Expression of MMP-9 and IL-6 mRNA in chondric and synovial tissues was detected in 30 cases with osteoarthritis group and 10 cases with normal control group by in situ hybridization(ISH). Results IOD of MMP-9 mRNA and corresponding protein in OA was significantly higher than normal control group (P<0.01), and there was difference between IOD of IL-6 mRNA and corresponding protein in OA and control group (P<0.05). There were positive correlations both between mRNA and corresponding protein in OA (r=0.924, 0.917; P<0.01). There were also significantly positive correlations both between IOD of MMP-9 and IL-6 in OA detected by ISH and IHC (r=0.426, P<0.05; r=0.444, P<0.01),respectively. Conclusion IL-6 may be up-regulate the expression of MMP-9 of articular chondrocytes and synoviocytes in OA, and both promote the biological behavior of OA such as occurrence,progress,etc. |
| Key words: osteoarthritis metalloproteinase-9 interleukin-6 in situ hybridization immunohistochemistry |
