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| CpG-ODN对小鼠骨髓造血系统辐射损伤的保护作用 |
| 张超,高福,崔建国,程赢,孙顶,周传丰,刘聪,李百龙,蔡建明* |
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| (第二军医大学海军医学系放射医学教研室，上海 200433) |
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| 摘要: |
| 目的探讨Toll样受体9（Toll-like recptor 9,TLR9）激动剂——含CpG基序的寡核苷酸（CpG-ODN）对小鼠骨髓造血系统辐射损伤的治疗作用。方法小鼠在照射后30 min、24 h、48 h连续腹腔注射CpG-ODN（50 μg/只），观察小鼠不同剂量照射后存活率的变化，检测特定时间内血液中白细胞和骨髓中有核细胞的含量及其骨髓病理切片，外源性脾结节集落形成数。结果CpG-ODN能提高照后小鼠的存活率，提高小鼠外周血白细胞和骨髓有核细胞数（P<0.01），缓解骨髓病理学的损伤，减少骨髓干细胞的死亡。结论CpG-ODN能减轻小鼠骨髓造血系统辐射损伤。 |
| 关键词: CpG-ODN Toll样受体9 辐射损伤 骨髓 造血系统 |
| DOI：10.3724/SP.J.1008.2011.0372 |
| 投稿时间：2011-04-06修订日期：2011-04-11 |
| 基金项目:国家自然科学基金（31070762），上海市自然科学基金（10ZR1437500）. |
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| Protective effect of CpG-ODN against irradiation injury of bone marrow hematopoietic system in mice |
| ZHANG Chao,GAO Fu,CUI Jian-guo,CHENG Ying,SUN Ding,ZHOU Chuan-feng,LIU Cong,LI Bai-long,CAI Jian-ming* |
| (Department of Radiation Medicine, Faculty of Navy Medicine, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| ObjectiveTo study the therapeutic effect of CpG-ODN, an agonist of Toll-like receptor 9 (TLR9), on irradiation injury of bone marrow hematopoietic system in mice. MethodsMice were treated with intraperitoneal injection of CpG-ODN (50 μg each) at 30 min, 24 h and 48 h after irradiation. The survival rates of animals were observed after irradiation with different doses, the numbers of white blood cell (WBC) and bone marrow nucleated cells within a certain period of time were observed, the bone marrow was pathologically studied, and the number of endogenous colony forming unit-spleen (endoCFU-S) was counted. ResultsOur results showed that intraperitoneal injection of CpG-ODN significantly improved the survival rate of mice and increased the numbers of peripheral WBC and bone marrow nucleated cells (P<0.01); moreover, it also ameliorated the pathological injury of the bone marrow and reduced the death of bone marrow stem cells.ConclusionIntraperitoneal CpG-ODN injection can ameliorate the irradiation injury of bone marrow hematopoietic system in mice. |
| Key words: CpG-ODN Toll-like receptor 9 radiation injuries bone marrow hematopoietic system |
