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| 大肠癌早期肝转移的血清特征蛋白及组织表达特异基因筛查 |
| 蔡相军1*,熊仁青1,王福荣1,王春喜2,王斌1,王克夫1,刘彩刚3,冷晗1 |
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| (1. 解放军202医院普通外科,沈阳 110003;2. 解放军301医院普通外科,北京 100853;3. 中国医科大学附属第一医院乳腺外科,沈阳 110001) |
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| 摘要: |
| 目的应用表面增强激光解吸电离飞行时间质谱(SELDI-TOF-MS)技术及RT2 ProfilerTM PCR Array Human Tumor Metastasis(PAHS-028A)芯片筛查早期肝转移患者血清特异性蛋白及相关肿瘤基因,为大肠癌肝转移的早期诊断提供理论依据。方法应用弱阳离子蛋白质芯片(WCX2)及SELDI-TOF-MS技术检测20例大肠癌和20例大肠癌早期肝转移患者血清中蛋白的相对含量。PCR基因芯片技术对原发灶和肝转移灶组织进行差异基因筛选。结果应用SELDI-TOF-MS的3组 |
| 关键词: 结直肠肿瘤 肝肿瘤 肿瘤转移 蛋白质陈列分析 基因芯片 |
| DOI：10.3724/SP.J.1008.2011.0856 |
| 投稿时间：2011-04-11修订日期：2011-07-05 |
| 基金项目:沈阳市科学技术计划项目(071995). |
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| Screening of serum proteomic patterns and tissue-specific genes for early liver metastasis of colorectal cancer |
| CAI Xiang-jun1*,XIONG Ren-qing1,WANG Fu-rong1,WANG Chun-xi2,WANG Bin1,WANG Ke-fu1,LIU Cai-gang3,LENG Han1 |
| (1. Department of General Surgery, No. 202 Hospital of PLA,Shenyang 110003, Liaoning, China;2. Department of General Surgery, No. 301 Hospital of PLA, Beijing 100853, China;3. Department of Breast Surgery, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China) |
| Abstract: |
| ObjectiveTo screen for the serum proteomic patterns and related genes in early liver metastasis of colorectal cancer by surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) and the RT2 ProfilerTM PCR Array Human Tum |
| Key words: colorectal neoplasms liver neoplasms neoplasm metastasis protein array analysis gene chips |
