| 摘要: |
| 目的研究姜黄素对小鼠肾小管上皮细胞体外再灌注损伤的保护作用及机制。方法采用Percoll溶液密度梯度离心法,分离并培养小鼠肾小管上皮细胞。培养的细胞分为空白对照组、缺氧再灌注组、姜黄素低剂量组(5 μmol/L)及高剂量组(25 μmol/L)。流式细胞仪分析细胞凋亡率；荧光定量PCR及蛋白质印迹法检测细胞内TLR4表达,蛋白质印迹法检测NF-κB p65磷酸化；荧光定量PCR检测各组细胞内IL-6、IL-1β、TNF-α和MCP-1 的mRNA水平,ELISA法检测培养液上清内上述因子的含量。结果分离的 |
| 关键词: 姜黄素 肾小管 上皮细胞 再灌注损伤 Toll样受体4 炎症 |
| DOI：10.3724/SP.J.1008.2011.0874 |
| 投稿时间：2011-04-12修订日期：2011-05-26 |
| 基金项目:上海市卫生局青年科研项目（2008Y081） |
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| Protective effect of curcumin against reperfusion injury of mouse renal tubular epithelial cells in vitro |
| WU Di-ying1,GUO Wen-yuan2*,ZHANG Hao-jie1,CAI Xu-dong1,XU Peng-jie1 |
| (1. Department of Nephrology, Lihuili Hospital, Ningbo University, Ningbo 315040, Zhejiang, China;2. Department of Organ Transplantation, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China) |
| Abstract: |
| ObjectiveTo evaluate the protective effect of curcumin against reperfusion injury of mouse renal tubular epithelial cells in vitro and the related mechanisms. MethodsThe renal tubular epithelial cells (TECs) were isolated by an improved Percoll density gr |
| Key words: curcumin kidney tubules epithelial cells reperfusion injury Toll-like receptor 4 inflammation |
