| 摘要: |
| 目的 探讨人参皂苷Rg1对1-甲基-4-苯基吡啶离子(1-methyl-4-phenylpyridinum, MPP+)诱导的PC12细胞凋亡是否具有保护作用。方法 采用MPP+诱导的具有多巴胺能神经元特性的PC12细胞凋亡作为帕金森病(Parkinson disease,PD)的体外模型。实验分正常对照组、MPP+损伤组、人参皂苷Rg1(10、20、50 μmol/L)3个浓度预处理组。用MTT法测定细胞活性,流式细胞术检测细胞凋亡,TUNEL法检测细胞凋亡断裂的DNA片段,蛋白质印迹法分析细胞色素C(cytochrome C, Cyt C)蛋白含量。结果 10、20、50 μmol/L人参皂苷Rg1对MPP+诱导的PC12细胞具有一定的保护作用。与MPP+损伤组 [(52±4.7)%] 相比,10、20、50 μmol/L人参皂苷Rg1预处理细胞活力分别上升为(64±3.4)%、(72±5.2)%、(83±6.2)% (P<0.05或P<0.01)。经流式细胞术检测，正常组、MPP+损伤组、人参皂苷Rg1预处理组(10、20、50 μmol/L)细胞凋亡率分别为1.8%、44.5%、32.9%、21.1%和14.2%。人参皂苷Rg1预处理后,细胞断裂的DNA片段明显减少。另外,蛋白质印迹分析也显示人参皂苷Rg1可抑制MPP+诱导的Cyt C的过表达。结论人参皂苷Rg1对MPP+诱导的细胞凋亡具有浓度依赖性的保护作用,其保护机制可能与下调线粒体内Cyt C的过表达有关。 |
| 关键词: 人参皂苷Rg1 PC12细胞 细胞凋亡 细胞色素C类 神经保护药 |
| DOI：10.3724/SP.J.1008.2011.0965 |
| 投稿时间：2011-04-28修订日期：2011-06-02 |
| 基金项目: |
|
| Neuroprotective effects of ginsenoside Rg1 on 1-methyl-4-phenylpyridinum-induced apoptosis in PC12 cells |
| YE Xiao-li1*,LI Xiao-feng2 |
(1.Department of Cadres, No. 323 Hospital of PLA, Xi'an 710046, Shaanxi,China
2. Laboratory of Integrated Traditional Chinese Medicine and Western Medicine of Elderly Encephalopathy, Research Center of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China
*Corresponding author.) |
| Abstract: |
| ObjectiveTo explore the neuroprotective effect of ginsenoside Rg1 against PC12 cell apoptosis induced by 1-methyl-4-phenylpyridinum(MPP+). MethodsMPP+-induced apoptosis in PC12 cells, with the characteristics of dopaminergic neuron, were taken as the model of Parkinson disease in vitro. The cells were divided into control group, MPP+ group and 3 ginsenoside Rg1 pretreatment groups (concentrations 10, 20, and 50 μmol/L). MTT assay was used for detecting the cell viability, FCM for apoptosis ratio, TUNEL enzyme labelling for DNA fragment of the cell nuclear, and Western blotting analysis for cytochrome C protein.ResultsGinsenoside Rg1 (10, 20, and 50 μmol/L) showed protective effect against MPP+-induced PC12 cells injury. Compared with MPP+-treated cells(\[52±4.7\]%), pretreatment with 10, 20, and 50 μmol/L ginsenoside Rg1 increased the cell viability to (64±3.4)%, (72±5.2)% and (83±6.2)%, respectively (P<0.05 or P<0.01). FCM analysis indicated that apoptosis rates decreased by ginsenoside Rg1 pretreatment, with the apoptosis rates in the control, MPP+ and 3 ginsenoside Rg1 groups (10, 20, 50 μmol/L) being 1.8%, 44.5%, 32.9%, 21.1% and 14.2%, respectively. We also found that ginsenoside Rg1 pretreatment greatly decreased DNA fragment of PC12 cells. Western blotting analysis indicated that the cytochrome C was depressed by the ginsenoside Rg1 pretreatment. ConclusionGinsenoside Rg1 can protect PC12 cells against MPP+-induced apoptosis in a concentration-dependent manner, which may be closely related to down-regulation of cytochrome C over-expression in the mitochondria. |
| Key words: ginsenoside Rg1 PC12 cells apoptosis cytochromes C neuroprotective agents |
