| 摘要: |
| 目的 探讨TRPV1基因敲除后外周痛觉的改变。方法 采用甩尾热痛仪和弗莱毛测痛法测量TRPV1基因敲除型及野生型雌性小鼠的热和机械痛阈,并进行比较。结果 热刺激后,TRPV1基因敲除型雌性小鼠较野生型雌性小鼠的甩尾潜伏期延长[(3.59±0.65) s vs (2.19±0.24) s,P<0.05],两组间机械痛阈值差异无统计学意义[(1.71±0.57) g vs (2.13±0.81) g,P>0.05]。结论 在生理条件下,TRPV1受体介导热刺激痛,与机械刺激痛无明显相关。 |
| 关键词: TRPV阳离子通道 基因敲除小鼠 痛阈 |
| DOI：10.3724/SP.J.1008.2011.01013 |
| 投稿时间：2011-05-27修订日期：2011-07-01 |
| 基金项目: |
|
| Changes of peripheral pain threshold in transient receptor potential vanilloid 1 knockout female mice |
| GONG Qi△,LU Zhan-ying△,YU Li-hua,MA Bei* |
(Department of Physiology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China
△Co-first authors.
*Corresponding author.) |
| Abstract: |
| ObjectiveTo investigate the changes of peripheral pain threshold after knockout of transient receptor potential vanilloid 1(TRPV1) gene. MethodsTail-Flick Analgesia Meter and von-Frey hair were used to determine the peripheral thermal and mechanical thresholds in TRPV1 knockout and wild-type female mice, and the results of the two groups were compared. ResultsThe Tail-Flick latency in TRPV1 knockout mice was significantly prolonged after hot stimulation compared with that in the wild-type group(\[3.59±0.65\] s vs \[2.19±0.24\] s, P<0.05).There was no significant change in responses to von-Frey hair stimuli between the two groups(\[1.71±0.57\] g vs \[2.13±0.81\] g,P>0.05). ConclusionIt is suggested that TRPV1 receptor mediate thermal stimuli response under physiological condition, and has no noticeable influence on mechanical stimuli response. |
| Key words: TRPV cation channels knockout mice pain threshold |
