| 摘要: |
| 目的 研究灯盏乙素对脂多糖(lipopolysaccharide ,LPS)诱导BV2细胞株炎症细胞因子表达的影响,探讨灯盏乙素抗炎作用机制。 方法 通过荧光素酶报告基因检测核转录因子NF-κB活性,并利用LPS刺激使BV2细胞和通过RT-PCR和蛋白免疫法检测TNF-α和IL-1β的mRNA及蛋白含量,研究灯盏乙素的抗炎作用。实验分为正常组、LPS刺激(1 μg/ml)的模型组、灯盏乙素组(0.1、1、10和100 μg/ml)。 结果 灯盏乙素预处理(1~100 μg/ml)可抑制LPS引起pNF-κB 293的核转录因子NF-κB激活(P<0.05或P<0.01); 灯盏乙素预处理(1~100 μg/ml)能够抑制LPS引起BV2的TNF-α和IL-1β mRNA和蛋白含量升高(P<0.01)。 结论 灯盏乙素具有抗炎作用,其抗炎作用与抑制核转录因子NF-κB、抑制炎症细胞因子表达有关。 |
| 关键词: 灯盏乙素 炎症 细胞因子类 肿瘤坏死因子 白介素 |
| DOI：10.3724/SP.J.1008.2011.01235 |
| 投稿时间：2011-06-08修订日期：2011-10-10 |
| 基金项目: |
|
| Scutellarin inhibits LPS-induced pro-inflammatory cytokine expression in BV2 cells |
| MAO Jun-qin1,MENG Hui1*,ZHAO Dan1,WANG Run1,ZHENG Yue2 |
(1. Department of Pharmacy, No.85 Hospital of PLA, Shanghai 200052, China;
2. Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
*Corresponding author.) |
| Abstract: |
| Objective To study the effects of scutellarin on LPS-induced pro-inflammatory cytokine expression in BV2 cells, so as to explore the anti-inflammatory mechanism of scutellarin. Methods The experiment was divided into 5 groups, namely, a normal control group, a model control group (stimulated by LPS), and three Scutellarin treatment groups (0.1, 1, and 100 μg/ml). NF-κB activity was examined by Luciferase Reporter Gene Assay. BV2 cells were treated with LPS ; expressions of TNF-α and IL-1β mRNA and protein were examined by real-time PCR and Western blotting analysis, respectively. Results Scutellarin treatment greatly inhibited LPS-induced NF-κB activation in pNF-κB 293 cells. The treatment also noticeably inhibited LPS-induced expression of TNF-α and IL-1β in BV-2 cells. Conclusion Scutellarin has anti-inflammatory effect, which maybe related to the inhibition of NF-κB-mediated pathway and inhibition of pro-inflammatory cytokine expression. |
| Key words: scutellarin inflammation cytokines tumor necrosis factor interleukin |
