| 摘要: |
| 目的 检测胃癌组织中肝素酶基因(heparanase,HPA)及可能靶向其表达的microRNAs(miRNAs)的表达水平。方法 应用实时定量PCR检测HPA在胃癌组织中的表达变化；根据miRNAs与HPA 3′非翻译区(3′-UTR)序列的结合情况,运用生物信息学方法 筛选出可能靶向HPA的miRNAs,并利用实时定量PCR检测这些miRNAs在胃癌组织中的表达水平。结果 HPA在胃癌组织中表达水平较癌旁组织和正常组织高；生物信息学分析显示,miR-18b、miR-137、miR-502和miR-299-3p可能结合于HPA的3′-UTR上,抑制其表达；实时定量PCR检测结果 显示,胃癌组织miR-18b、miR-137、miR-502和miR-299-3p的表达均较癌旁组织和正常组织低。结论 胃癌组织HPA高表达,而可能靶向HPA的miR-18b、miR-137、miR-502和miR-299-3p低表达,提示上述靶向HPA的miRNAs可能参与了胃癌的发生发展。 |
| 关键词: 肝素酶 胃肿瘤 微RNAs 基因表达 生物信息学 |
| DOI：10.3724/SP.J.1008.2012.0200 |
| 投稿时间：2011-07-05修订日期：2012-01-16 |
| 基金项目: |
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| Expression of microRNAs targeting heparinase in gastric cancer |
| ZHANG Yan1,YU Ji-yao2,GUO Shi-wei3,WANG Yu-hong1* |
(1. Department of General Surgery, Clinical College of Navy Medicine, Second Military Medical University, Beijing 100048, China
2. Department of Pathology, Clinical College of Navy Medicine, Second Military Medical University, Beijing 100048, China
3. Department of General Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
*Corresponding author.) |
| Abstract: |
| Objective To investigate the expression of heparinase (HPA) and miRNAs that may regulate HPA expression in the gastric cancer tissue. Methods The expression levels of HPA were determined by real-time PCR in the gastric cancer tissues. Based on the binding of miRNAs with the 3′ untranslated region (3′-UTR) of HPA, we screened for miRNAs targeting HPA using bioinformatics method and determined the expression levels of these miRNAs in the gastric cancer tissues using real-time PCR. Results The expression of HPA was higher in the gastric cancer tissues compared with those in the tumor-adjacent and normal tissues. Bioinformatics analysis showed that miR-18b,miR-137,miR-502,and miR-299-3p might inhibit HPA expression by binding to its 3′-UTR. Real-time PCR analysis revealed lower expression levels of miR-18b,miR-137,miR-502,and miR-299-3p in the gastric cancer tissues compared with in the adjacent and normal tissues. Conclusion HPA is highly expressed in the gastric cancer tissues; miR-18b, miR-137, miR-502 and miR-299-3p, which may target HPA, are lowly expressed, indicating miRNAs targeting HPA may participate in the carcinogenesis of gastric cancer. |
| Key words: heparinase stomach neoplasms microRNAs gene expression bioinformatics |
