| 摘要: |
| 靶标范围的不断拓展是指数式富集的配体系统进化(SELEX)技术和其他随机文库技术(如噬菌体表面展示技术等)的显著特征之一。经过20多年的发展，SELEX的靶标已经拓展到包括金属离子、各种有机小分子、药物、蛋白质等生物大分子、致病微生物、活细胞和组织等在内的多种形式，以细胞和组织为靶标的SELEX筛选策略在肿瘤靶向诊断和治疗的研究中也得到了初步应用，本文综述了SELEX技术的靶标拓展情况。 |
| 关键词: SELEX适体技术 随机寡核酸文库 靶标 适配体 |
| DOI：10.3724/SP.J.1008.2012.00432 |
| 投稿时间：2011-07-07修订日期：2012-03-12 |
| 基金项目:重庆市科委科技攻关计划项目(CSTC,2010AC5132)和重庆市卫生局医学科研项目(2010-1-55,2009-2-122). |
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| Expanding targets for systematic evolution of ligands by exponential enrichment (SELEX) |
| XU Fa-liang1*,XIAO Jue2 |
(1. Treatment Centre of Breast Diseases, Chongqing Cancer Hospital and Institute, Chongqing400030, China
2. Department of Pathology, Chongqing Cancer Hospital and Institute, Chongqing 400030, China
*Corresponding author.) |
| Abstract: |
| The continuous expanding of targets is a distinct character of systematic evolution of ligands by exponential enrichment (SELEX) technique and other random library technologys (such as phage display technology). Over the past 20 years the targets of SELEX have expanded to various metal ions, organic molecules, drugs, proteins and biomacromolecules, pathogenic bacteria, live cells and tissue slide. The SELEX screenings using live cells and tissue slide as targets have been preliminarily applied for studying targeted diagnosis and treatment of cancers. This paper reviews the expansion of the targets of SELEX technique. |
| Key words: SELEX aptamer technique random oligonucleotide library target aptamer |
