| 摘要: |
| 目的 分析人源可诱导共刺激分子(ICOS)可溶性融合蛋白(ICOSIg)与鼠源不成熟树突状细胞(DCs)是否发生特异性结合及其一般生物学特性。 方法 通过流式细胞术结合特异性抗体检测了解ICOSIg与不成熟DCs的结合作用;以Annexin Ⅴ/PI双染色、活细胞染料CSFE和CCK-8研究ICOSIg对DCs的细胞毒性作用;以 掺入法研究ICOSIg对于混合淋巴细胞反应的阻断作用。 结果 ICOSIg可结合小鼠DCs表面的ICOSL,ICOSIg不引起DCs早期和晚期凋亡,不影响DCs细胞的增殖,可以抑制不同基因小鼠脾细胞的混合淋巴细胞反应。 结论 本实验室构建的体外表达的ICOSIg具有较强的生物学活性,对鼠DCs无明显的生物学毒性作用,首次从实验角度证实人源ICOSIg可以特异性结合小鼠不成熟DCs表面配体ICOSL,可以用于进一步探讨ICOSL/ICOS共刺激通路的免疫作用。 |
| 关键词: 可诱导共刺激分子 树突细胞 重组融合蛋白质类 抑制 |
| DOI：10.3724/SP.J.1008.2011.01165 |
| 投稿时间：2011-09-08修订日期：2011-10-27 |
| 基金项目:国家自然科学基金青年基金(81102249), 中国人民解放军南京军区2010年医学课题(10MA057), 江西省卫生厅2011年度科技计划项目(20112058). |
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| Biological effect of human-derived recombinant ICOSIg on mouse immature dendritic cells |
| XU Jin-song1,WU Yu1,SHEN Qian2,TANG Gu-sheng2* |
(1. Department of Respiratory Diseases, No. 94 Hospital of PLA, Nanchang 330002, Jiangxi, China;
2. Department of Laboratory Diagnosis, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
*Corresponding author.) |
| Abstract: |
| Objective To analyze whether human-derived ICOSIg can bind specifically to ICOSL on mouse immature dendritic cells(DCs) and to explore its biological functions. Methods The binding of ICOSIg to immature DCs was observed by FCM. The cytotoxic effect of ICOSIg on DCs was examined by Annexin Ⅴ/PI, CFSE staining, and CCK-8 kit. thymine incorporation was used to analyze the blocking effect ICOSIg on mixed lymphocyte reaction (MLR). Results Human-derived soluble fusion protein ICOSIg could bind to ICOSL on mouse bone marrow-derived immature DCs and inhibited the MLR, but it neither induced early or late apoptosis of DCs nor affected their proliferation. Conclusion Human-derived ICOSIg constructed in this study has a potent biological function; it has no toxic effect against mouse immature DCs. It is demonstrated that human-derived ICOSIg can sepecifically bind to ICOSL on mouse immature DCs. |
| Key words: inducible costimulator dendritic cells recombinant fusion proteins inhibition |
