| 摘要: |
| 目的探讨单羧酸转运蛋白-1(MCT1)、CD147、CD44 mRNA在非小细胞肺癌(NSCLC)中的表达及其在NSCLC发生、发展和预后中的意义。方法取经病理确诊的56例NSCLC患者的癌及癌旁组织(距肿瘤边缘>5 cm)、20例肺良性病变、7例正常肺组织组织标本,采用实时荧光定量RT-PCR检测MCT1、CD147、CD44 mRNA表达,并分析癌组织中三者表达的相关性及与临床病理特征的关系。结果(1)MCT1、CD147、CD44 mRNA在肺癌组织中的表达量高于癌旁组织、正常肺组织和肺良性病变组织,差异均有统计学意义(P均＜0.05)；(2) 癌组织中MCT1、CD147、CD44 mRNA三者表达具有相关性(P<0.05)；(3) MCT1、CD147、CD44 mRNA的表达与NSCLC患者的性别、年龄、是否吸烟、肿瘤大小、pTNM分期均无关,但与肿瘤的组织类型(P均<0.05)、淋巴结转移(P均<0.05)有关；MCT1、CD44 mRNA的表达还与肿瘤分化程度有关(P均<0.05)。结论在NSCLC患者组织中MCT1、CD147、CD44 mRNA表达上调,它们可能参与肿瘤的发生、发展,有望成为NSCLC诊断和预后评价的指标。 |
| 关键词: 非小细胞肺癌 单羧酸转运蛋白1 CD147 CD44 |
| DOI：10.3724/SP.J.1008.2012.00528 |
| 投稿时间：2011-12-20修订日期：2012-04-10 |
| 基金项目:云南省卫生厅科技计划项目(2011WS0005). |
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| Expression of monocarboxylate transporter 1, CD147 and CD44 mRNA in non-small cell lung cancer: correlation and significance |
| ZHU Chao-nan1,SHI Yun2*,WANG Ping1,ZHOU Jian-hong1,PENG Hao1,DIAO Jian-you2 |
(1. Department of Cardiothoracic Surgery, The Affiliated Kunhua Hospital of Kunming Medical College, The First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan, China
2. Department of Cardiothoracic Surgery, Kunming General Hospital, PLA Chengdu Military Area Command,Kunming 650032, Yunnan, China
*Corresponding author.) |
| Abstract: |
| ObjectiveTo study the expression of monocarboxylate transporter 1(MCT1), CD147, and CD44 mRNA in non-small cell lung cancer(NSCLC), and its role in the development, progression and prognosis of NSCLC. MethodsFifty-six NSCLC specimens and the adjacent tissues (more than 5 cm from the tumors), 20 specimens of benign pulmonary diseases and 7 specimens of normal pulmonary tissues were included in the present study. The expression of MCT1, CD147, and CD44 mRNA was examined by real-time quantitative RT-PCR in the above specimens. The relationship of their expression with the clinical and pathological features of NSCLC patients was analyzed. Results(1)The expression rates of MCT1, CD147, and CD44 mRNA in NSCLC specimens were significantly higher than those in the adjacent tissues, normal pulmonary tissues, and benign pulmonary disease tissues (all P<0.05). (2) The expression of MCT1, CD147, and CD44 in NSCLC tissue was significantly correlated with each other(P<0.05). (3) The expression of MCT1, CD147, and CD44 mRNA in NSCLC tissue was not correlated with age, gender, smoking, site and dimension of lesion, or pTNM stage, and was correlated with the histological types (all P<0.05) and lymph node metastasis of NSCLC(all P<0.05). In addition, we also found that MCT1 and CD44 expression was correlated with the differentiation degree of NSCLC(all P<0.05). ConclusionThe expression of MCT1, CD147, and CD44 mRNA is up-regulated in the NSCLC specimens. MCT1, CD147 and CD44 may participate in the development and progression of NSCLC, and they may also act as markers for diagnosis and prognosis of NSCLC. |
| Key words: non-small cell lung cancer monocarboxylate transporters one CD147 CD44 |
