| 摘要: |
| 罗布麻叶为夹竹桃科多年生草本植物罗布麻(Apocynum venetum L.)的干燥叶[1]。罗布麻中含黄酮类化合物、生物碱、强心苷、氨基酸和微量元素[2],用于治疗高血压病、心悸头晕、慢性支气管炎、高血脂等病症[3]。罗布麻遍布于我国华北、西北及黄河流域的盐碱沙荒地区,资源丰富[4]。罗布麻化学成分复杂,挥发油是其主要的活性成分之一[5]。罗布麻叶的挥发油由于产地、品种、生长环境、栽培变异等因素的影响而有较大区别[6]。因此,全面表征罗布麻挥发性化学成分,筛选不同来源罗布麻的化学生物标志物,对于罗布麻的质量评价十分有必要。本研究采用顶空气相色谱-质谱(GC-MS)联用技术对来源于新疆、吉林、天津、江苏4个不同产地的20批罗布麻的挥发性成分进行鉴别以及含量测定,并且结合主成分分析(PCA)等多元统计技术的组合分析方法,筛选出对于质量评价起主要影响的化学差异标志物,为综合评价不同产地的罗布麻药材提供一定的实验依据。 |
| 关键词: 罗布麻；挥发油；气相色谱-质谱法 多元分析 质量评价 |
| DOI：10.3724/SP.J.1008.2012.00924 |
| 投稿时间：2011-12-26修订日期：2012-04-23 |
| 基金项目: |
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| GC-MS combined with multivariate statistical method for evaluating quality of Apocynum venetum |
| LIANG Shan-shan1,ZHAO Liang1,ZHANG Hai1,TAN Guang-guo2,CHAI Yi-feng2,ZHANG Guo-qing1* |
(1. Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China
2. Department of Drug Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
*Corresponding author.) |
| Abstract: |
| Objective To analyze the chemical constituents of essential oil extracted from Apocynum venetum and screen the index components to evaluate essential oil in samples from different habitats. Methods Head-space-GC／MS was employed to prepare essential oil from Apocynum venetum，with HP-5 quartz capillary column，extraction temperature 90℃ ，hold 30 min，injection volume 1．5 mL .The datas were disposed by multivariate statistical analysis to screen the index components. Result 80 volatile components were analyzed and quantified. The system reproducibility was good .7 index volatile components were screened and the total content of the index components in samples from Jiangsu is higher than others. Conclusion The total contents of the index components were distinct in Apocynum venetum from different habitats. A rapid，simple and reliable method using HS-GC/MS and multivariate statistical methed was developed to analyze the volatile components which can be an alternative for quality evaluation for TCMs such as Apocynum venetum． |
| Key words: Apocynum venetum L. essential oil gas-chromatography-mass spectrometry multivariate analysis quality evaluation |
