| 摘要: |
| 目的研究槐定碱对心肌梗死大鼠心功能及心肌细胞超微结构的影响。方法采用结扎冠状动脉左前降支方法制备SD大鼠心肌梗死模型,随机分为模型对照组和槐定碱低、中、高(5、10、20 mg/kg)剂量组,另设假手术组,每组10只。实验结束后测定左室舒张末压(LVEDP),左室内压最大上升、下降速率(±dp/dtmax)和心脏质量指数(HM/BM),用透射电子显微镜观察各组大鼠心肌细胞超微结构的变化。结果与假手术组相比,模型对照组的±dp/dtmax降低,LVEDP升高,HM/BM升高(P均<0.01)；与模型对照组相比,加药各组的±dp/dtmax升高,LVEDP降低,HM/BM降低,除低剂量组-dp/dtmax外,余者与模型对照组相比差异均有统计学意义(P<0.01，P<0.05)；电镜结果显示假手术组大鼠心肌细胞肌丝排列整齐,线粒体结构正常。模型对照组大鼠心肌细胞肌丝溶解,线粒体肿胀,嵴模糊不清,有显著的病理学改变;槐定碱低剂量组的肌丝溶解有轻微恢复,中、高剂量组肌丝溶解现象消失,肌丝排列整齐。结论槐定碱能改善心肌梗死大鼠的心功能,减轻心肌细胞超微结构的损伤。 |
| 关键词: 槐定碱 心肌梗死 心脏功能 超微结构 |
| DOI：10.3724/SP.J.1008.2012.00470 |
| 投稿时间：2012-01-11修订日期：2012-03-31 |
| 基金项目:军队医药卫生“十一五”科研项目(06MB202). |
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| Effects of sophoridine on cardiac function and myocardial ultrastructure of rats with myocardial infarction |
| LU Hong,SHEN Ya-feng,CAO Mi,SONG Xiao-wei,TANG Ying,YANG Yong-ji* |
(Department of Biophysics, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China
*Corresponding author.) |
| Abstract: |
| ObjectiveTo explore the effects of sophoridine on cardiac function and myocardial ultrastructure of rats with myocardial infarction. MethodsThe SD rat model of myocardial infarction (MI) was obtained by permanent ligation of the left anterior descending coronary artery (LAD), and the model rats were randomly divided into model group (rats with MI), low-dose group (5 mg/kg sophoridine), middle-dose group (10 mg/kg sophoridine), and high-dose group (20 mg/kg sophoridine); a sham operated group was also included. Each group had 10 rats. The elevated left ventricular end-diastolic pressure (LVEDP), ±dp/dtmax and heart mass/body mass (HM/BM) were determined after experiment, and the changes of myocardial ultrastructure were observed by transmission electron microscope. ResultsCompared with the sham operated group, ±dp/dtmax in MI group was significantly decreased, and LVEDP and HM/BM were remarkably increased (all P<0.01). Compared with MI group, ±dp/dtmax in the 3 sophoridine groups were significantly increased, and LVEDP and HM/BM were significantly decreased (P<0.01, P<0.05), except for -dp/dtmax in the low-dose sophoridine group. Transmission electron microscope found that the myofilaments of myocardial cells in the sham operated group were well arranged, with normal mitochondria. The myofilaments were dissolved in MI rats, with swollen mitochondria and blurred cristae, showing distinct pathological changes. There was a slight improvement in myofilament dissolution in the low-dose sophoridine group, and the myofilament dissolution disappeared in the middle- and high-dose group, with well arranged myofilaments. ConclusionSophoridine can improve the cardiac function of MI rats, and has protective effects on ultrastructure of myocardial cells. |
| Key words: sophoridine myocardial infarction heart function ultrastructure |
