| 摘要: |
| 目的用两种方法建立家兔铜绿假单胞菌肺部感染模型,并根据病理学、影像学等指标进行评价、比较。方法48只健康清洁级新西兰大白兔随机平均分成A、B、C、D 4组,A组采用经皮气管穿刺法,B组采用经鼻喷雾吸入法，分别隔日接种铜绿假单胞菌反复感染家兔,C、D组施以无菌生理盐水分别作为穿刺对照组和吸入对照组。接种后隔日1次行胸部CT扫描。待家兔感染死亡后取肺脏进行病理学检查。从病理学、影像学和实验室检查等方面验证动物模型并对经皮气管穿刺法和经鼻喷雾吸入法两种建模方法进行比较评价。结果(1)病理学指标:接种感染后早期死亡动物肺部均有不同程度的水肿、出血、结节样脓肿、实变表现,镜下可见肺组织内大量中性粒细胞浸润,灶状脓肿形成；感染死亡较晚动物的肺部实变逐渐减少,以局部肺不张、纤维增生、肉芽肿形成、淋巴细胞浸润为主要表现。(2)影像学指标:A、B两组胸部CT均表现为双侧多发斑片状模糊影,部分可见实变及脓肿灶。A组于接种后第5天CT影像上首先发现病变,B组接种后第7天发现病变。结论使用经皮气管穿刺法和喷雾吸入法接种铜绿假单胞菌至新西兰兔肺部,均可成功建立兔铜绿假单胞菌急慢性病程演变的原发肺部感染模型；两种方法在可操作性、可控性、可适性等方面各有优点。 |
| 关键词: 铜绿假单胞菌 细菌性肺炎 动物模型 方法 |
| DOI：10.3724/SP.J.1008.2012.00829 |
| 投稿时间：2012-01-15修订日期：2012-06-05 |
| 基金项目:国家自然科学基金(30970800), 上海市科委重点项目(10411952800,10JC1418100). |
|
| Two methods for establishing animal model of pulmonary Pseudomonas aeruginosa infection |
| LI Bin△,ZHU Dong-qing△,YU Hong*,LIU Hong-chao,LIU Shi-yuan |
(Department of Imaging, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
△Co-first authors.
*Corresponding authors.) |
| Abstract: |
| ObjectiveTo establish rabbit model of pulmonary Pseudomonas aeruginosa (PA) infection by two different methods and make evaluation and comparison from the pathology, imaging and other aspects. MethodsTotally 48 healthy New Zealand rabbits were randomly divided into 4 groups (n=12): group A, B, C and D. Group A was inoculated with PA suspension via percutaneous tracheal puncturing; Group B was inoculated with PA suspension via spraying inhaling; and group C and D were treated with sterile saline suspension serving as puncturing control and inhaling control, respectively. PA inoculation was done in the four groups every other day, and chest CT scan was performed every other day after inoculation. Pulmonary pathological examination was done after the death of the animals. The two methods were evaluated and compared concerning the pathological, imaging, and laboratory findings. Results(1) Pathological findings in group A and B: For animals of early death, there were pulmonary edema of different degrees, hemorrhage, nodule-like abscess, and consolidation; moreover, severe neutrophil infiltration and abscess-like lesions in lung tissue were observed under the microscope. For animals of later death, the consolidation was gradually relieved and the main pathological findings included local atelectasis, fibrosis, granuloma formation, and lymphocyte infiltration. (2) Radiographic findings in group A and B: Chest CT showed bilateral multiple patchy opacities, with consolidation and abscess seen in some areas. Lesions on CT were found at 5 days after inoculation in group A and at 7 days after inoculation in group B.ConclusionRepeatedly inoculating PA suspension via both percutaneous tracheal puncturing and spraying inhaling can successfully establish rabbit model of pulmonary PA infection, and each method has their own advantages in performance, controllability, and adaptability. |
| Key words: Pseudomonas aeruginosa |
