| 摘要: |
| 目的 探讨津力达颗粒对糖尿病大鼠胰岛B细胞的保护作用。方法 采用高脂饮食,链脲佐菌素腹腔一次性注射诱发SD大鼠糖尿病模型,随机分为模型组、津力达药物(0.75、1.5、3.0 g/kg)组、α-硫辛酸组、胰岛素组、二甲双胍组。根据分组分别给予不同的药物,胰岛素组采用皮下注射,其余药物均灌胃给药。给药2个月后测定体质量，空腹血糖(FBG)、胰岛素(FINS)、糖化血红蛋白(HbA1c)水平，超氧化物歧化酶（SOD）、谷胱甘肽（GSH）活性,白介素（IL）-1β、TNF-α和丙二醛（MDA）含量,并取胰尾组织行胰岛素免疫组化染色观察。结果 与模型组相比,其他各组FBG、HbA1c等均降低(P<0.05, P<0.01)；津力达高剂量(3.0 g/kg)组SOD、GSH活性升高(P<0.01),IL-1β、TNF-α、MDA含量降低(P<0.01),胰岛素免疫阳性染色的面积增加。结论 津力达颗粒对受损的糖尿病大鼠胰岛B细胞具有保护作用。 |
| 关键词: 津力达颗粒 糖尿病 胰岛素分泌细胞 氧化性应激 |
| DOI：10.3724/SP.J.1008.2012.00385 |
| 投稿时间：2012-01-17修订日期：2012-03-22 |
| 基金项目:国家重点基础研究发展计划(“973”计划,2005CB523304)． |
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| Protective effect of Jinlida granules on islet β cells in diabetes mellitus rats |
| SHI Jing-li,WU Ying,SONG Yu-ping,HAN Chong,LIU Zhi-min* |
(Department of Endocrinology, Changzheng Hospital, Second Military Medical University, Shanghai200003, China
*Corresponding author.) |
| Abstract: |
| Objective To investigate the protective effect of Jinlida granules on islet β cells in diabetes mellitus rats. Methods Diabetic models were induced by high-fat diet and intraperitoneal injection of streptozotocin (STZ ) in SD rats. The study was divided into model group, Jinlida granule group (0.75, 1.5, 3.0 g/kg), α-lipoic acid group, insulin group, and metformin group. Each group was given corresponding drugs, with insulin given by subcutaneous injection and other drugs given by intragastric administration. The body weight, fasting blood glucose(FBG), fasting insulin (FINS), glycosylated hemoglobin (HbA1c), the activities of superoxide dismutase (SOD) and glutathione(GSH), and the contents of malondialdehyde(MDA), IL-1β and TNF-α were examined 2 months later. The pancreatic tissues were immunostained for insulin. Results Compared with the model group, the levels of FBG and HbA1c were decreased in other groups(P<0.05, P<0.01). The activities of SOD and GSH were significantly increased (P<0.01) and the levels of MDA, IL-1β and TNF-α were significantly decreased (P<0.01) in high-dose (3.0 g/kg) Jinlida granule group, with increased islet area as shown by pathological staining. Conclusion Jinlida granule has protective effect against STZ-induced islet β cell injury in diabetic rats. |
| Key words: Jinlida granule diabetes mellitus islet β cells oxidative stress |
