| 摘要: |
| 目的 运用高效液相-高分辨飞行时间质谱(HPLC-TOF/MS)技术对中药复方扶正平消胶囊化学成分进行鉴别。方法 色谱分离采用Agilent Eclipse plus C18 (4.6 mm×250 mm,5 μm) 色谱柱；流动相为乙腈和0.1％甲酸水溶液,梯度洗脱:0~90 min,5%A~95%A；柱温25℃；流速1 ml/min,柱后分流比为31。质谱定性采用飞行时间质谱,电喷雾离子源(ESI),正负离子模式共同监测,质量数扫描范围m/z 100~1 100。结果 共鉴别出扶正平消胶囊中247种的化学成分,其中正离子模式下168个、负离子模式下103个、正负离子均有响应24个,并对成分进行了药材归属。结论 建立了一种基于HPLC-TOF/MS技术对中药复方扶正平消胶囊中的化学成分进行鉴别的有效方法,为其质量控制及体内的深入研究奠定了基础。 |
| 关键词: 扶正平消胶囊 化学成分 鉴别 高效液相-高分辨飞行时间质谱 |
| DOI：10.3724/SP.J.1008.2012.00770 |
| 投稿时间：2012-03-27修订日期：2012-05-07 |
| 基金项目:上海市药学会科研基金(2011-YY-03-01). |
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| HPLC-TOF/MS in identification of chemical constituents of Fuzhengpingxiao Capsule |
| ZHAO Liang, TIAN Wen-jun, Lü Lei, ZHANG Hai, WANG Xin-xia, ZHANG Guo-qing* |
(Department of Pharmacy, Eastern Hepatobiliary Hospital, Second Military Medical University, Shanghai 200438, China
*Corresponding author.) |
| Abstract: |
| ObjectiveTo use high-performance liquid chromatography-time of flight mass spectrometry (HPLC-TOF/MS) for analyzing the chemical constituents of Fuzhengpingxiao Capsule. MethodsThe separation was performed on a Agilent Eclipse plus C18 reverse phase column (4.6 mm×250 mm, 5 μm)．The mobile phase consisted of water containing 0.1% formic acid and acetonitrile was used as gradient elute．The flow rate was 1 ml/min, the post-column split ratio was 31, and the temperature of column was 25℃. Time-of-flight mass spectrometer(TOF/MS) and electrospray ion source (ESI) was employed for qualitative analysis under both positive and negative ion mode, and mass scan range was m/z 100-1 100. ResultsA total of 247 major chemical constituents were identified in Fuzhengpingxiao Capsule by HPLC-TOF/MS, including 168 in positive mode, 103 in negative mode, and 24 in both. The source herb of the components were identified. ConclusionAn efficient HPLC-TOF/MS approach has been established for studying the chemical constituents in Fuzhengpingxiao Capsule, which paves a way for the quality control and further in vivo studies of the preparation. |
| Key words: Fuzhengpingxiao capsule chemical constituents identification high-performance liquid chromatography-time of flight mass spectrometry |
