| 摘要: |
| 目的 探讨AKR1B10和GPC-3联合应用对提高肝细胞癌(HCC)免疫组化诊断敏感性和特异性的价值。方法 制备75例HCC组织芯片作为训练集,进行AKR1B10和GPC-3免疫组化检测,建立Logistic回归诊断模型,以此构建ROC曲线(受试者工作特征曲线),利用其曲线下面积(AUC)对单个指标和联合指标的敏感性和特异性进行评估。将Logistic回归诊断模型用于200例HCC的测试集中,检测其有效性。 结果 训练集中,AKR1B10和GPC-3的AUC分别为0.773和0.800,联合诊断后的AUC提高至0.931；AKR1B10和GPC-3的敏感性分别为56%和61.3%,特异性均为98.7%,两者联合后的敏感性提高至88.0%,特异性为97.3%。测试集中,AKR1B10联合GPC-3对HCC诊断的敏感性和特异性分别为97.0%和96.5%。结论 AKR1B10联合GPC-3明显提高HCC免疫组化诊断的敏感性和特异性,可在常规病理检查中合理组合使用。 |
| 关键词: 肝细胞癌 免疫组织化学 醛酮还原酶1B10 磷脂酰蛋白聚糖3 诊断 |
| DOI：10.3724/SP.J.1008.2012.00625 |
| 投稿时间：2012-05-02修订日期：2012-06-01 |
| 基金项目:国家自然科学基金(30921006,81072026),上海市科委重点项目(10411951000). |
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| Application of AKR1B10 combined with GPC-3 in immunohistochemical diagnosis of hepatocellular carcinoma |
| JIN Guang-zhi1△,GU Yi-jin1△,YU Hao2 ,CONG Wen-ming1* |
(1. Department of Pathology, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China
2. Department of Laparoscopy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China
△Co-first authors.
*Corresponding author.) |
| Abstract: |
| Objective To explore the value of AKR1B10 combined with GPC-3 in improving the sensitivity and specificity of immunohistochemical diagnosis of hepatocellular carcinoma (HCC). Methods The microarray including 75 HCC and adjacent tissues was subjected to immunohistochemistry detection of AKR1B10 and GPC-3 expression. A Logistic regression diagnostic model was established using the results of tissue microarray (training group). The ROC curves (the receiver-operating characteristic curve) and area under the curve (AUC) were used to evaluate the sensitivity and specificity of AKR1B10, GPC-3 or their combination. The Logistic regression diagnostic model was validated with 200 HCC and adjacent tissues (testing group). Results For the training group, the AUC values of AKR1B10, GPC-3, and AKR1B10 combined with GPC-3 were 0.773, 0.800, and 0.931, respectively. The sensitivity of AKR1B10 and GPC-3 were 56% and 61.3%, respectively, and their specificity was both 98.7%. AKR1B10 combined with GPC-3 yielded a sensitivity of 88.0% and a specificity of 97.3%.For the testing group, sensitivity and specificity of AKR1B10 combined with GPC-3 were 97.0% and 96.5%, respectively. Conclusion AKR1B10 combined with GPC-3 can greatly improve the sensitivity and specificity of HCC immunohistochemical diagnosis, and it should be used when necessary in addition to the routine pathological assessment. |
| Key words: hepatocellular carcinoma immunohistochemistry aldo-keto reductase family 1, member B10 glypican 3 diagnosis |
