| 摘要: |
| 目的 观察不同退变程度的人颈椎间盘髓核(nucleus pulposus, NP)组织中性别决定基因组9(SOX9)、Ⅱ型胶原α1(COL2A1)的表达差异,并探讨此差异在人颈椎间盘退变(cervical intervertebral disc degeneration, CIDD)发生发展中的意义。 方法 收集在我院行手术治疗的36例颈椎病或颈椎外伤患者的NP组织及术前颈椎MRI(T2加权像正中矢状位)资料,采用Miyazaki分级系统评估退变程度。用免疫组织化学法观察SOX9、COL2A1在NP组织中的表达定位,用荧光定量(FQ) RT-PCR、蛋白质印迹分析法检测SOX9、COL2A1在mRNA及蛋白水平上的表达量,分析其表达水平与CIDD程度间的关系,初步探究SOX9和COL2A1间的相互作用。结果 SOX9在NP细胞胞核中表达,COL2A1在细胞外基质中表达；随着CIDD退变程度的加重,两者的mRNA和蛋白表达水平均逐渐降低；且以SOX9表达量为自变量(X)、COL2A1表达量为应变量(Y)的直线回归方程为Y=0.923X-0.059(P<0.001)。结论 随着CIDD程度加重,SOX9与COL2A1基因表达水平逐渐降低,两者下降趋势相近,SOX9正向调控COL2A1的表达,提示SOX9与COL2A1基因功能改变可能是CIDD的分子机制之一。 |
| 关键词: 颈椎 椎间盘退变 髓核 SOX9 COL2A1 |
| DOI：10.3724/SP.J.1008.2013.00117 |
| 投稿时间：2012-06-07修订日期：2012-12-10 |
| 基金项目:上海市科委基金(11JC1415600),军队“十一五”医药卫生科研基金(06MA163). |
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| Expression of SOX9 and COL2A1 gene in human cervical intervertebral disc nucleus pulpusus and its significance |
| CHENG Yong-jun,QI Ke,HE Ping,SONG Qing-xin,WANG Kun,SHEN Hong-xing* |
(Department of Orthopaedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
*Corresponding author.) |
| Abstract: |
| Objective To observe the expression of SRY(sex determining region Y)-box 9 (SOX9) and collagen type Ⅱalpha 1 (COL2A1) in human cervical intervertebral disc nucleus pulposus (NP) tissues of various degeneration degrees, so as to discuss the role of their expression during cervical intervertebral disc degeneration(CIDD). Methods Totally 36 human cervical intervertebral disc specimens and their pre-operation MRI findings were obtained from surgical patients or patients with cervical trauma, and they were classified into five grades according to the Miyazaki grading system. The expression of SOX9 and COL2A1 was examined by fluorescence quantitative RT-PCR, Western blotting analysis and immunohistochemistry staining. The relationship between SOX9, COL2A1 expression and the degrees of CIDD was analyzed, and the relationship between SOX9 and COL2A1 was also discussed. Results Both SOX9 and COL2A1 were expressed in NP tissues, with SOX9 seen in the nucleus and COL2A1 in the extracellular matrix. The expression of SOX9 and COL2A1 mRNA and protein gradually decreased as Miyazaki Ⅰprogressed to Miyazaki Ⅴ.We also found a linear relationship between COL2A1 (Y) and SOX9 (X) expression: Y=0.923X-0.059 (P<0.001). Conclusion The expression of SOX9 and COL2A1 mRNA decrease with the increase of CIDD severity, with SOX9 and COL2A1 mRNA having a similar decreasing tendency. SOX9 positively regulate COL2A1 expression, indicating that changes of SOX9 and COL2A1 gene might be one of the molecular mechanisms of CIDD. |
| Key words: cervical vertebrae intervertebral disc degeneration nucleus pulposus SOX9 COL2A1 |
