| 摘要: |
| [摘要] 目的 研究血浆microRNA-208b（miR-208b）和microRNA-145（miR-145）在急性冠脉综合征（acute coronary syndrome，ACS）中的表达变化及临床意义。方法 收集50例ACS患者为ACS组，包括不稳定心绞痛（unstable angina，UAP）20例和急性心肌梗死患者（acute myocardial infarction，AMI）30例为亚组；16例健康体检者作为对照组。收集ACS患者首次出现胸痛症状（5±1.4 h）（n=50），12h（n=6）及7d（n=6）不同时间点的血浆。采用实时荧光定量PCR（quantitative real-time PCR， qRT-PCR）测定其血浆miR -208b及miR-145的表达水平，分析时间变化趋势及与CTnI的相关性。结果（1）ACS患者出现胸痛后（5±1.24 h），对照组与UAP亚组血浆miR208b表达水平为0，只有AMI亚组血浆miR208b的表达水平为（442.16±174.38），ACS患者血浆miR208b表达（265.70±256.24）高于对照组（P <0.05）；AMI亚组血浆miR208b的表达高于UAP亚组(P <0.05)。（2）患者出现胸痛后（5±1.24 h），ACS患者血浆mi145表达水平为（0.21±0.12），对照组血浆miR145表达水平为（1.00±0.00），ACS患者血浆miR145表达低于对照组（P <0.05）；AMI亚组患者血浆miR145的表达水平为（0.19±0.10），UAP亚组miR145表达水平为（0.25±0.14），两组差异无统计学意义（P >0.05）。（3）检测ACS患者在（5±1.4 h）血浆miR208b达到最高峰，血浆miR-145达到最低峰，与对照组相比表达变化有统计学意义（P<0.05），7d后回归基线。CTnI6h开始升高，12h达到高峰，7d回归基线。血浆miR208b及miR-145时间变化趋势与CTnI具有一致性。(4) 血浆miR208b的表达水平与cTnI成正相关（r=0.833，P <0.05）；血浆miR145的表达水平与cTnI无相关性（r=0.352，P >0.05）；（5）miR-208b，血浆miR145的表达水平与WBC无相关性（r=-0.243,-0.083，P> 0.05)。
结论 血浆miR208b在ACS组血浆表达升高而miR145表达降低，尤其在AMI组变化更明显，可能作为急性冠脉综合征早期诊断及鉴别诊断的敏感指标。
结论 血浆miR208b在ACS组血浆表达升高而miR145表达降低，尤其在AMI组变化更明显，可能作为急性冠脉综合征早期诊断及鉴别诊断的敏感指标。
结论 血浆miR208b在ACS组血浆表达升高而miR145表达降低，尤其在AMI组变化更明显，可能作为急性冠脉综合征早期诊断及鉴别诊断的生物标志物。 |
| 关键词: 急性冠脉综合征 miR-208b miR-145 血浆 |
| DOI：10.3724/SP.J.1008.2013.00109 |
| 投稿时间：2012-06-16修订日期：2012-11-16 |
| 基金项目:河南省科技厅重点攻关项目(122102310226). |
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| Plasma expression of microRNA-208b and microRNA-145 in acute coronary syndrome patients and its clinical relevance |
| HE Fu-cheng1,ZHAO Xue1,GAO Feng2,WANG Xi3,SUN Li1,ZHU Qing-hua1 |
(1. Laboratory Department,the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China
2. Henan Academy of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450052, Henan, China
3. Department of Cardiovasology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China) |
| Abstract: |
| [ABSTRACT] Objectives To investigate the expression of plasma miR -208b, miR -145 and clinical significance in acute coronary syndrome（ACS）patients. Methods 16 healthy people and 50 ACS patients,including 30 patients with AMI and 20 patients with UAP were enrolled. The plasma samples from ACS patients were obtained within(5±1.4h)（n=50），12h（n=6）and 7d（n=6） after the onset of ACS symtoms. Examing the expression levels of plasma miR -208b and miR -145 by quantitative real-time PCR technology (qRT-PCR) and analyzing the correlation between miR-208b,miR145 expression levels and cTnI,WBC .Results (1) The miR-208b level in plasma from ACS group was higher than that of control group (P <0.05) and the plasma miR208b level in acute myocardial infarction was higher than that of unstable angina group (P <0.05) within (5±1.4h); (2)The miR-145 level in plasma from ACS group was lower than that of in control group(P<0.05) and there is no significant difference of the plasma miR145 expression level in AMI group and UAP group (P >0.05) within (5±1.4h) (3)CTnI which was significantly increased at 6h after ACS remained increased until 12h,and it was restored to the normol value at 7day;both miR208b and miR145 were at their peak values at 6h,and retuned to the control levels at 7d (4)There was positively correlation between of plasma miR208b and cardiac troponin I（cTnI）. (r=0.833, P <0.05)，there was no correlation between plasma miR145 and CTnI（r=0.352，P > 0.05).（5）There was no correlation between of plasma miR208b,145 and WBC（r=-0.243,-0.083，P > 0.05). Conclusion Plasma miR208b is up-regulated and plasma miR145 is down-regulated in ACS. These results suggested that plasma miR208b and miR-145 can be used as a sensitive indicator for ACS.diagnosis. |
| Key words: acute coronary syndrome miR-208b miR-145 plasma |
