| 摘要: |
| [摘要] 目的 建立高效液相色谱法测定肝力保胶囊中苦参碱及槐定碱含量。方法 使用Agilent NH2 (4.6 mm×250 mm, 5μm)，流动相：1%磷酸:无水乙醇:乙腈=10：22：68，流速0.8 ml/min，柱温25℃，检测波长210nm，测定15批肝力保胶囊中苦参碱及槐定碱含量。结果 苦参碱在5.21-156.3 μg/ml （r=1），槐定碱在5.29-158.7 μg/ml（r=0.9999）浓度范围内呈良好线性关系，苦参碱回收率为98.73% (RSD=3.67 %)，槐定碱回收率为100.10%（RSD=2.24%）。15批肝力保胶囊中苦参碱含量在9.04-13.4 1 mg/粒之间，槐定碱含量在6.48-15.62 mg/粒之间。结论 该方法简便、准确，重复性好，可用于肝力保胶囊的质量控制。 |
| 关键词: 高效液相色谱法 苦参碱 槐定碱 肝力保胶囊 含量测定 |
| DOI：10.3724/SP.J.1008.2012.001267 |
| 投稿时间：2012-06-25修订日期：2012-10-25 |
| 基金项目: |
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| Determination of matrine and sophoridine contents in ganlibao capsule by High Performance Liquid Chromatography |
| wanghui |
| () |
| Abstract: |
| Objective To establish a high performance liquid chromatography approach for determining matrine and sophoridine contents in Ganlibao capsule. MethodsAgilent NH2 (4.6 mm×250 mm, 5 μm) column was used with 1% phosphoric acidethanolacetonitrile (V/V/V=102268) as the mobile phase. The flow rate was 0.8 ml/min, and the temperature was at 25℃.The detection wavelength was set at 210 nm. Results The calibration curve of matrine showed a good linearity in the range of 5.21-156.3 μg/ml (r=1) and that of sophoridine in the range of 5.29-158.7 μg/ml (r=0.999 9). The equations of linear regression of matrine and sophoridine were Y=9.586 1X+1.003 8 and Y=7.604 3X-5.920 1, respectively. The recovery was 98.73% (RSD=3.67%) for matrine and 100.10% (RSD=2.24%) for sophoridine. The matrine content of 15 batches of Ganlibao capsule was (30.13±0.19)-(44.70±0.17) mg/g and the sophoridine content was (21.60±0.25)-(52.07±0.11) mg/g. Conclusion The present method is simple, accurate, and reproducible, and it can be used for the quality control of Ganlibao capsule. |
| Key words: HPLC matrine sophoridine ganlibao capsule determination |
