| 摘要: |
| 目的 比较血糖正常大鼠和糖尿病大鼠全脑缺血再灌注后海马CA1区Ku70和Bax的表达变化,探讨Ku70和Bax在糖尿病加重脑缺血再灌注神经损伤中的作用。方法 雄性SD大鼠72只,随机分为假手术(SO)组、血糖正常全脑缺血再灌注(NCI)组和糖尿病全脑缺血再灌注(DCI)组。DCI组采用链脲佐菌素诱导联合改良的Pulsineli 4血管阻断(4-VO)法制作糖尿病全脑缺血再灌注模型,NCI组不用链脲佐菌素诱导,其余处理同DCI组；SO组仅暴露血管。分别在缺血再灌注1、6、24和48 h应用光镜观察海马CA1区神经细胞形态变化,免疫组织化学和免疫印迹法检测海马CA1区Ku70蛋白表达水平,免疫组织化学方法检测海马CA1区Bax蛋白表达水平。结果 NCI 组大鼠海马CA1区细胞结构破坏,各时间点存活神经元密度与SO组比较均减少(P<0.05)；DCI 组大鼠海马CA1区神经元结构损伤加重,各时间点存活神经元密度较NCI组降低(P<0.05)。NCI 组1 h和6 h时间点Ku70表达较SO组增强(P<0.05),24 h和48 h表达较SO组降低(P<0.05)；DCI组各时间点Ku70表达均较NCI组降低(P<0.05)。NCI 组各时间点Bax表达均较SO组增强(P<0.05),DCI组各时间点Bax表达均较NCI组增强(P<0.05)。结论 糖尿病可加重全脑缺血再灌注后神经损伤,其机制可能与进一步加重Ku70低表达和Bax高表达有关。 |
| 关键词: 糖尿病 脑缺血 再灌注损伤 Ku70 Bax |
| DOI： |
| 投稿时间：2012-10-20修订日期：2013-04-01 |
| 基金项目:河北省自然科学基金(H2012401007),河北省卫生厅重点课题(ZD2010106). |
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| Expression of Ku70 and Bax in hippocampus CA1 following cerebral ischemia/reperfusion injury in diabetic rats |
| ZHAO Ya-ning,WANG Zhu,LI Jian-min*,CHANG Xue-you,JING Li-wei,CHEN Chang-xiang,LI Shu-xing |
(Institute of Neurosurgery, Rehabilitation College, Hebei United University, Tangshan 063000, Hebei, China
*Corresponding author.) |
| Abstract: |
| Objective To compare the expression of Ku70 and Bax in hippocampus CA1 between normal rats and diabetic rats following cerebral ischemia/reperfusion,so as to explore the roles of Ku70 and Bax in aggravating cerebral ischemia/reperfusion injury in diabetes. Methods Totally 72 male SD rats were divided into 3 groups randomly: sham operated (SO) group, normoglycemia global cerebral ischemia/reperfusion (NCI) group, and diabetic global cerebral ischemia/reperfusion (DCI) group. The rats in DCI group were treated by streptozocin (STZ) and improved Pulsinelli’s four-vessel occlusion method to establish diabetic global cerebral ischemia/reperfusion model. And animals in NCI group were not given STZ, and other treatments were similar to those in the DCI group. Animals in the SO group only had blood vessels exposed. Changes of neuron pathology in hippocampus CA1 were observed by H-E staining under light microscopy at 1, 6, 24, and 48 h after ischemia/reperfusion; expression of Ku70 was detected by immunohistochemistry and Western blotting analysis, and expression of Bax was detected by immunohistochemistry. Results The neuron structure in hippocampus CA1 of rats was damaged in NCI group, and the density of survival neurons was significantly lower than that in the SO group at all studied time points(P<0.05); the neuron structure damage in DCI group was more severe, and the density of survival neurons was significantly lower than that of the NCI group at all studied time points(P<0.05). The expression of Ku70 at 1 and 6 h in NCI group was significantly higher than that in the SO group (P<0.05), and that at 24 and 48 h was significantly lower than that in the SO group(P<0.05); Ku70 expression in DCI group was significantly lower than that in the NCI group at all studied time points(P<0.05). Bax expression in NCI group was significantly higher than that in the SO group at all studied time points(P<0.05), and that in DCI group was significantly higher than that in the NCI group at all studied time points (P<0.05). Conclusion Diabetes can aggravate global cerebral ischemia/reperfusion injury, which may be related to aggravated Ku70 expression decrease and Bax expression increase. |
| Key words: diabetes mellitus brain ischemia reperfusion injury Ku70 Bax |
