| 摘要: |
| 目的 观察A型肉毒毒素(BTX-A)对大鼠食管下括约肌离体肌条自发性收缩及乙酰胆碱(ACh)、P物质(SP)引发的收缩增强是否存在抑制作用,并探讨其作用机制。方法 剪取食管下括约肌制备肌条并随机分为对照组、BTX-A组、ACh组、ACh+BTX-A组、ACh+阿托品组、SP组、SP+NK1受体拮抗剂(APTL)-SP组、SP+BTX-A组,采用Biolap420E生物机能实验系统记录肌条在不同条件下的收缩变化。结果 BTX-A降低食管下括约肌自发性收缩张力及振幅(P<0.05)；ACh可增强食管下括约肌收缩张力及振幅(P<0.01),而BTX-A、阿托品均可抑制ACh的增强效应(P<0.01)；SP可增强食管下括约肌收缩张力(P<0.01),其增强效应均可被BTX-A、APTL-SP所抑制(P<0.01)。结论 ACh、SP可增强食管下括约肌的收缩能力,而BTX-A可抑制其引发的收缩增强效应。 |
| 关键词: A型肉毒毒素 乙酰胆碱 P物质 食管下括约肌 |
| DOI：10.3724/SP.J.1008.2013.00164 |
| 投稿时间：2012-11-28修订日期：2013-01-04 |
| 基金项目:河南省教育厅自然科学研究课题(2011C310012) ,漯河医学高等专科学校自然科学研究课题(2010S11). |
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| Inhibitory effect of botulinum toxin type A against exogenous ACh- and substance P-induced contraction of rat lower esophageal sphincter in vitro |
| LI Chao-yan1,YU Hai-ying1,ZHOU Yuan-yuan1,REN Liang1,CHU Xian-xiang2* |
(1. School of Basic Medical Sciences, Luohe Medical College, Luohe 462002, Henan, China
2. Department of Anatomy, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450000, Henan, China
*Corresponding author.) |
| Abstract: |
| Objective To study whether botulinum toxin type A (BTX-A) can inhibit the spontaneous and acetylcholine (ACh)- or substance P (SP)-induced contraction of rat lower esophageal sphincter in vitro, and to discuss the related mechanism. Methods The lower esophagus muscle strips were taken from Sprague-Dawley rats and were randomly divided into control group, BTX-A group, ACh group, ACh+BTX-A group, ACh+Atropine group, SP group, SP+APTL-SP group and SP+BTX-A group. The contractile graph of the muscle strips was recorded by physiological experimental system Biolap-420E. Results BTX-A significantly decreased the spontaneous contractile tension and amplitude in the lower esophageal sphincter(P<0.05). ACh significantly enhanced the contractile tension and amplitude in the lower esophageal sphincter (P<0.01), which could be significantly inhibited by both BTX-A and Atropine (P<0.01). SP significantly enhanced the contractile tension in the lower esophageal sphincter (P<0.01), which could be significantly inhibited by both BTX-A and APTL-SP (P<0.01). Conclusion ACh and SP can enhance the spontaneous contractility of lower esophageal sphincter. BTX-A can inhibit ACh- and SP-induced enhancement of lower esophageal sphincter contraction. |
| Key words: botulinum toxin type A acetylcholine substance P lower esophageal sphincter |
