| 摘要: |
| 目的 探讨微小RNA分子miR-214对食管鳞癌细胞Eca109侵袭能力的影响及其可能的分子机制。方法 根据人源miR-214序列合成双链模拟物。通过脂质体转染将miR-214模拟物分子导入食管鳞癌Eca109细胞中,转染无关miRNA模拟物作为对照。采用定量PCR法检测细胞中成熟miR-214分子水平,以Matrigel包被的Transwell实验测定侵袭细胞率,通过蛋白质印迹法检测细胞中E-钙黏蛋白(E-cadherin)的表达,利用流式细胞术检测E-cadherin阳性细胞率。结果 Eca109细胞转染miR-214模拟物48 h后,其成熟miR-214水平较对照组明显上升(P<0.01)；细胞侵袭能力较对照组降低(P<0.05)。同时,miR-214转染组的E-cadherin表达水平及E-cadherin阳性细胞率较对照组下降(P<0.05)。 结论 miR-214可能通过抑制细胞上皮间质转化(EMT)的方式抑制食管鳞癌细胞的侵袭能力。 |
| 关键词: 微RNAs 食管肿瘤 鳞状细胞癌 肿瘤侵润 上皮间质转化 |
| DOI：10.3724/SP.J.1008.2013.00001 |
| 投稿时间：2012-12-01修订日期：2013-01-04 |
| 基金项目:国家自然科学基金(30872552). |
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| Effect of miR-214 on invasive capacity of esophageal squamous carcinoma cell line Eca109 |
| HAN Qing-qi1,YAO Feng2,XU Tong-yi1,XU Zhi-yun1* |
(1. Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
2. Department of Thoracic Surgery, Shanghai Chest Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200030, China
*Corresponding author.) |
| Abstract: |
| Objective To investigate the effect of miR-214 on the invasive capacity of esophageal squamous carcinoma cell line Eca109, and to explore the possible molecular mechanism. Methods We prepared miR-214 double-stranded mimic and transfected it into Eca109 cells with Lipofectamine 2000. Eca109 cells transfected with nonsense miRNA mimics were taken as control. The expression of mature miR-214 was determined by qPCR. The capacity of cell invasion was determined by Matrigel-coated Transwell assay. E-cadherin protein expression and the percentage of E-cadherin positive cells were examined by Western blotting analysis and flow cytometry, respectively. Results The expression level of mature miR-214 in the miR-214 mimic transfection group was significantly higher than that in the control group 48 h after transfection (P<0.01). Eca109 cells transfected with miR-214 mimic showed a significantly lower cell invasive capacity compared to that of cells transfected with control miRNA mimic (P<0.05). Moreover, E-cadherin protein expression and the ratio of E-cadherin positive cells in miR-214 mimic transfection group were both significantly lower than those in the control group (P<0.05). Conclusion Our data suggest that miR-214 may inhibit the invasive capacity of esophageal squamous carcinoma cells by repressing the epithelial-mesenchymal transition. |
| Key words: microRNAs esophageal neoplasms squamous cell carcinoma neoplasm invasiveness epithelial-mesenchymal transition |
