| 摘要: |
| 目的 运用代谢组学方法对黄芩素作用白假丝酵母菌引起的胞内代谢差异进行表征，寻找潜在的生物标志物并探讨相关的药物作用机制。 方法 采用气相色谱-质谱方法测定黄芩素作用前后白假丝酵母菌胞内的代谢指纹谱，利用多元统计分析方法比较黄芩素给药组与对照组的代谢物谱差异，筛选出可能的潜在生物标志物，并作出相关代谢途径的可能解释。 结果和结论 经比较分析，共筛选得到20个潜在生物标志物，这些重要的差异代谢物主要参与了氨基酸代谢、三羧酸循环、磷脂代谢和氧化应激等相关通路。 |
| 关键词: 代谢组学 气相色谱-质谱法 多元分析 黄芩素 白假丝酵母菌 |
| DOI：10.3724/SP.J.1008.2013.00184 |
| 投稿时间：2012-12-16修订日期：2013-01-15 |
| 基金项目: |
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| GC-MS based metabolic analysis of baicalein-treated Candida albicans |
| WU Hai-tang1,LI Xiang2,WANG Tian-qi1,CHEN Xiao-fei1,CAO Ying-ying3,ZHU Zhen-yu1* |
(1. Department of Drug Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433，China
2. No. 62380 Troop of PLA, Beijing 100071, China
3. New Drug R&D Center, School of Pharmacy, Second Military Medical University, Shanghai 200433，China
*Corresponding author.) |
| Abstract: |
| Objective To conduct a metabolic analysis of the intracellular metabolism in baicalein-treated Candida albicans, so as to search for possible biomarkers and to discuss the mechanism of baicalein. Methods GC-MS analysis was used to obtain the metabolic fingerprint of C. albicans before and after treatment with baicalein, and multivariate statistical analysis was used to identify the differences in intracellular metabolites between the treated and control groups, so as to search for the possible biomarkers and discuss the possible relevant metabolic pathways. Results and conclusion Twenty metabolites were screened out and selected as potential biomarkers, and they were primarily involved in oxidative stress, citrate cycle, lipid metabolism and amino acid metabolism. |
| Key words: metabolomics gas chromatography-mass spectrometry multivariate analysis baicalein Candida albicans |
