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| 青蒿琥酯引起骨髓瘤细胞RPMI8226 G2/M期阻滞 |
| 王素云1*,刘志妙2,郝洪岭1,成志勇3,杨晓阳1,李杰1,王瑞仓1,李燕1,杨洁1,袁军1,王超1 |
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(1. 河北省人民医院血液内科, 石家庄 050001
2. 石家庄丰益肛泰医院外科, 石家庄 050000
3. 保定市第一医院血液内科, 石家庄 071000
*通信作者) |
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| 摘要: |
| 目的 研究青蒿琥酯对人多发性骨髓瘤细胞系RPMI8226细胞周期的影响及可能机制。方法 分别以浓度为0、25、50 μg/mL的青蒿琥酯作用于RPMI8226细胞,用透射电镜观察细胞变化,流式细胞术检测细胞周期分布,蛋白质印迹法检测细胞周期相关蛋白cyclin B1和p34cdc2的变化。结果 50 μg/mL青蒿琥酯处理48 h后,多数细胞显示出凋亡细胞的特征性形态。随着青蒿琥酯浓度增加,G0/G1期细胞的比例逐渐下降(P<0.05),G2/M期细胞的比例逐渐上升(P<0.05),细胞明显阻滞于G2/M期。细胞周期蛋白cyclin B1表达水平随青蒿琥酯浓度的增加而增加,p34cdc2表达水平随青蒿琥酯浓度的增加而降低。结论 青蒿琥酯能将RPMI8226细胞阻滞于G2/M期,其机制可能与cyclin B1的升高及p34cdc2的降低有关。 |
| 关键词: 青蒿琥酯 多发性骨髓瘤 细胞周期 细胞周期蛋白B1 CDC2蛋白激酶 |
| DOI： |
| 投稿时间：2013-01-09修订日期：2013-04-07 |
| 基金项目:河北省科技厅科技支撑计划项目(12276104D-1). |
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| Artesunate induced G2/M phase cell cycle arrest in multiple myeloma RPMI8226 cells |
| WANG Su-yun1*,LIU Zhi-miao2,HAO Hong-ling1,CHENG Zhi-yong3,YANG Xiao-yang1,LI Jie1,WANG Rui-cang1,LI Yan1,YANG Jie1,YUAN Jun1,WANG Chao1 |
(1. Department of Hematology, Hebei General Hospital, Shijiazhuang 050051, Hebei, China
2. Department of Surgery, Fengyigangtai Hospital of Shijiazhuang, Shijiazhuang 050000, Hebei, China
3. Department of Hematology, the First Hospital of Baoding, Baoding 071000, Hebei, China
*Corresponding author.) |
| Abstract: |
| Objective To investigate the effect of artesunate on the cell cycle of human multiple myeloma RPMI8226 cells and explore the related molecular mechanisms. Methods RPMI8226 cells were treated with 0, 25, and 50 μg/mL artesunate. The morphology change of cells was observed under transmission electron microscope, the cell cycle distribution was detected by flow cytometry, and the expression of cyclin B1 and p34cdc2 protein was examined by Western blotting analysis. Results After treated with 50 μg/mL artesunate for 48 h, most RPMI8226 cells showed characteristic morphology of apoptosis. With the increase of artesuate concentration, the proportion of RPMI8226 cells in G0/G1 phase was significantly decreased (P<0.05) and that of cells in G2/M phase was significantly increased (P<0.05), suggesting that artesunate induced noticeable G2/M arrest. Cyclin B1 level was increased and the p34cdc2 level was decreased with the increase of artesuate concentration. Conclusion Artesunate can induce G2/M arrests in RPMI8226 cells, which may be related to the increased cyclin B1 expression and decreased p34cdc2 expression. |
| Key words: artesunate multiple myeloma cell cycle cyclin B1 CDC2 protein kinase |
