| 摘要: |
| 目的 探索早年慢性应激对青春期病理性攻击大鼠空间学习记忆能力，及对海马脑源性神经营养因子(BDNF)、5-羟色胺(5-HT)水平的影响。方法 将20只雄性、出生后21 d 的Sprague-Dawley大鼠分为实验组、对照组,每组各10只。实验组大鼠采用孤养、昼夜颠倒、非奖赏性挫败、预激惹刺激、居住-入侵攻击等多种早年持续性应激方法持续到青春期。然后采用居住-入侵攻击实验检测两组青春期大鼠的攻击性,水迷宫实验观察其空间学习记忆能力,运用免疫组化方法检测大鼠海马内BDNF和5-HT水平。结果 (1)水迷宫实验:实验组大鼠在总路程、穿越目标区域次数、逃避潜伏期3项指标上与对照组差异有统计学意义(P<0.05),而中心区域路程/总路程比值与对照组比较差异无统计学意义(P>0.05)。(2)免疫组化结果显示:与对照组比较,实验组大鼠海马内BDNF和5-HT免疫阳性神经元数量均减少(P<0.05)，BDNF和5-HT表达降低。结论 BDNF和5-HT可能参与了青春期病理性攻击大鼠海马空间学习记忆能力的调控,并在空间学习记忆方面起着重要调控作用。 |
| 关键词: 脑源性神经营养因子 血清素 攻击 空间学习记忆 早年慢性应激 青春期 |
| DOI： |
| 投稿时间：2013-03-26修订日期：2013-06-09 |
| 基金项目:重庆市自然科学基金(CSTC,2011jjA10104),重庆医科大学国家自然科学预研资助项目(NSFYY201111). |
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| Effect of early life chronic stress on spatial learning and memory and hippocampus brain-derived neurotrophic factor and 5-HT in puberty pathological aggression rats |
| ZHANG Yan1,LEI Li1,HU Hua1*,QU Yuan1,L Xiao-juan1,QIN Guang-cheng2,CHEN Li-xue2 |
(1. Department of Psychiatry, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
2. Laboratory of Neurology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
*Corresponding author.) |
| Abstract: |
| Objective To explore the effect of early life chronic stress on spatial learning and memory and hippocampus brain-derived neurotrophic factor (BDNF) and 5-HT levels in puberty pathological aggression rats. Methods Twenty 21-day-old male rats were evenly randomized into 2 groups: experimental group and control group. Animals in the experimental group, from the early life till puberty, were given a series of stresses, including social isolation, reversed night and day, frustration test of non-reward, and resident intruder confrontations, etc. Resident intruder experiment was used to examine the aggressiveness of the animals; water maze experiments were performed to observe their spatial learning and memory. Immunohistochemistry method was used to test the expression of BDNF and 5-HT in the hippocampus. Results Morris water maze test showed that the total distance of the experimental group was significantly longer than that of the control group (P>0.05), and the experimental group had significantly less crossing times of hidden platform and escape latency compared with the control group (P<0.05); the ratios of central area distance/total distance were similar in the two groups. Immunohistochemistry findings showed that BDNF and 5-HT positive neurons were significantly less in the hippocampus of experimental group compared with the control group (P<0.05), and hippocampal BDNF and 5-HT expression in the experimental group was decreased. Conclusion BDNF and 5-HT may participate in the regulation of spatial learning and memory in the puberty pathological aggression rats, and they may play an important regulating role in spatial learning and memory. |
| Key words: brain-derived neurotrophic factor serotonin aggression spatial learning and memory early life chronic stress puberty |
