| 摘要: |
| 目的 优化大鼠脊髓髓内胶质瘤模型的构建方法,并对模型进行评估。方法 Fischer大鼠髓内注射9L胶质瘤细胞悬液,对肿瘤细胞的植入量、植入部位、植入深度等进行筛选优化,根据筛选好的指标构建大鼠脊髓髓内胶质瘤模型,采用BBB(Basso, Beattie, and Bresnahan)评分量表评估大鼠下肢神经功能,采用高分辨率MRI检查及病理免疫组化方法检查模型大鼠肿瘤形成情况,评估模型构建效果。结果 大鼠脊髓内植入9L胶质瘤细胞悬液构建大鼠髓内胶质瘤模型的优化指标为:植入平面在脊柱T10水平,植入量为6 μL(1.0×105 /mL)细胞悬液,植入位置在硬脊膜平面下3 mm。植入9L胶质瘤细胞后2周左右大鼠出现明显的下肢神经功能障碍。影像学和病理学检查证实髓内有肿瘤细胞生长。结论 成功构建大鼠髓内胶质瘤模型,为后续研究奠定了基础。 |
| 关键词: 脊髓肿瘤；神经胶质瘤 动物模型 9L胶质瘤细胞 |
| DOI： |
| 投稿时间：2013-05-02修订日期：2013-06-27 |
| 基金项目:上海市科委实验动物专项基金(11140903802),上海市科委纳米专项基金(12nm0501201). |
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| Establishment and evaluation of intramedullary spinal cord glioma model in rats |
| CAO Yi-qun,ZHANG Yu-hui,LI Ya-nan,CHEN Xin,WU Xi,HAN Guo-sheng,YUE Zhi-jian*,LIU Jian-min* |
(Department of Neurosurgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
*Corresponding authors.) |
| Abstract: |
| Objective To modify the establishment of intramedullary spinal cord glioma model in rats and to evaluate the established model. Methods Fischer rats were injected with 9L glioma cell suspension into the spinal cord. The optimal amount of implanted tumor cells, implantation sites, and depth were selected for establishment. Basso, Beattie, and Bresnahan (BBB) scale were used to evaluate the neuronal function of lower limbs of the rats; high resolution MRI and immunohistochemistry method were used to examine the tumor forming, so as to evaluate the established model. Results Our optimization parameters for establishing rat intramedullary spinal cord glioma models included: the amount of implanted 9L glioma cells was 6 μL (1.0×105/mL); the implantation site was at T10 level and the depth was 3 mm under the dural. The rats developed obvious lower limb neuronal dysfunction 2 weeks after implantation of 9L glioma cells. Pathological and MRI examination confirmed growth of intramedullary tumor cells. Conclusion Intramedullary spinal cord glioma models can be successfully established by implanting 9L glioma cells into the spinal cord of Fischer rats, which paving a way for future studys. |
| Key words: spinal cord neoplasms glioma animal models 9L glioma cells |
