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| 类鼻疽菌素是类鼻疽伯克霍尔德菌的主要毒力因子之一 |
| 马广强1,2,王倩1,朱金华1,叶荷平1,袁志明2,万红娇1* |
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(1. 江西中医药大学生命科学院医学免疫学与病原生物学学科组,南昌 330004
2. 中国科学院武汉病毒研究所,武汉 430071
*通信作者) |
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| 摘要: |
| 目的 研究类鼻疽菌素在类鼻疽伯克霍尔德菌(Bulkholderia pseudomallei,B. pseudomallei)致病性中的作用。方法 通过基因同源重组的方法突变类鼻疽菌素的主要合成酶基因mes C,检测野生菌和突变菌产生类鼻疽菌素能力的差异以及突变前后该菌对微生物(2株真菌:青霉菌、黑曲霉菌；2株细菌:粪链球菌、金黄色葡萄球菌)、线虫、小鼠的毒力差异。结果 突变菌的菌落形态发生了明显变化,失去了产生菌膜的能力,同时也失去了对微生物的抑制活性和对线虫、小鼠的致病性。结论 类鼻疽菌素可能是B. pseudomallei的主要毒力因子之一,在其致病性中起着重要的作用。 |
| 关键词: 类鼻疽伯克霍尔德菌 mes C基因 毒力因子类 类鼻疽菌素 |
| DOI： |
| 投稿时间：2013-05-22修订日期：2013-07-11 |
| 基金项目:国家高技术研究发展计划(“863”计划,2005AA219070). |
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| Melioidosin is a main virulent factor of Bulkholderia pseudomallei |
| MA Guang-qiang1,2,WANG Qian1,ZHU Jin-hua1,YE He-ping1,YUAN Zhi-ming2,WAN Hong-jiao1* |
(1. Medical Immunology and Pathogenic Group, College of Life Science, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Jiangxi, China
2. Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, Hubei, China
*Corresponding author.) |
| Abstract: |
| Objective To investigate the pathogenic role of melioidosin in pathogenicity of Burkholderia pseudomallei (B. pseudomallei). Methods A synthetase gene associated with melioidosin synthesis in B. pseudomallei was inactivated by homologous recombination. Melioidosin producting ability and the virulent effects of the wild-type and mutant B. pseudomallei upon microorganisms (Penicillium spp., Aspergillus niger spp., Streptococcus faecalis spp. and Staphyloccocus aureus spp.), C. elegans and BALB/c mice were compared. Results The mutant B. pseudomallei underwent notable morphology change, lost the ability to form bacterial membrane and the virulent effects to microorganisms, C. elegans and BALB/c mice. Conclusion It suggests that melioidosin is one of the main virulent factors of B. pseudomallei. |
| Key words: Burkholderia pseudomallei mes C gene virulence factors melioidosin |
