| 摘要: |
| 目的 对北沙参药材中的挥发油成分进行鉴别，并对比不同产地北沙参药材中的挥发油成分及含量的不同。方法 自2012年9月至2013年3月收集不同产地的北沙参药材共16批，然后进行研磨打粉。对北沙参粉末样品参照《中华人民共和国药典》附录方法提取挥发油。将挥发油样品进行气相色谱-质谱法(GC-MS)检测，对所得的质谱结果利用NIST 11.0数据库进行比对。结果和结论 从16个批次共48个北沙参挥发油样品中获得12个共有组分，都可作为北沙参挥发油的指标性成分,其中以法卡林醇为其挥发油中的主要成分。产于河北的3批样品中的挥发油成分及含量均较少。去皮加工的北沙参样品中挥发油总体含量比不去皮加工的北沙参少，并且发现二十碳五烯酸(eicosapentaenoic acid, EPA)仅存在于有皮的样品中。去皮加工方式会使一些有效成分的含量降低，如EPA，可能会影响北沙参的药用活性。 |
| 关键词: 北沙参 挥发油 气相色谱-质谱法 鉴定 法卡林醇 |
| DOI： |
| 投稿时间：2013-06-01修订日期：2013-07-22 |
| 基金项目:国家高技术研究发展计划(“863”计划, SS2013AA0903002). |
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| Identification of chemical constituents of volatile oil in coastal glehnia root |
| ZHANG Yi xin1,2,LU Xiao ling1,LIU Xiao yu1* |
(1. Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China
2. Biotechnology Group, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China
*Corresponding author.) |
| Abstract: |
| Objective To identify the chemical constituents of volatile oil from the Chinese traditional medicine coastal glehnia root and to compare the differences between coastal glehnia root of different locations.Methods A total of 16 batches of the coastal glehnia root were collected from several major production areas from September 2012 to March 2013, and then they were ground into powder. The volatile oil was extracted from the powder samples using the methods described in Chinese Pharmacopoeia Appendix. Gas chromatography-mass spectometry (GC-MS) was used to get the spectra of volatile oil of each sample and NIST 11.0 database was used to identify the chemical constituents of coastal glehnia root. Results and Conclusion From 16 batches of 48 coastal glehnia root volatile oil samples, we identified 12 common components. The 12 common chemical constituents can serve as the characteristic composition of volatile oil of the coastal glehnia root, and falcarinol is the major main chemical constituent. The three batches collected from Hebei province had fewer chemical components and lower contents. We also found that the coastal glehnia root samples with root bark had more volatile oil components and higher contents than the samples without bark; moreover, eicosapentaenoic acid was only found in the samples with root bark. Peeling the bark may reduce the contents of some volatile oils such as eicosapentaenoic acid, which may affect the medicinal activity of the coastal glehnia root. |
| Key words: Glehnia littoralis volatile oils gas chromatography-mass spectometry identification falcarinol |
