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| 前列腺癌相关长链非编码RNA在前列腺癌中的表达及生物学功能 |
| 胡晶晶1△,王富博2△,卢蕾1,王录美1,刘淑鹏1,汪佳祺1,任善成2,徐贵霞1,刘善荣1,孙颖浩2* |
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(1. 第二军医大学长海医院临床实验中心,上海 200433
2. 第二军医大学长海医院泌尿外科,上海 200433
△共同第一作者
*通信作者) |
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| 摘要: |
| 目的 探讨前列腺癌相关长链非编码RNA(prostate cancer related long non-coding RNA,PCRL)在前列腺癌中的表达情况及其潜在的生物学功能。方法 采用qRT-PCR检测PCRL在前列腺癌组织、癌旁组织及其他组织中的表达,同法检测并比较PCRL在雄激素依赖(LNCaP-AD) 与雄激素非依赖(LNCaP-AI) 前列腺癌细胞和正常前列腺上皮细胞(RWPE-1)中的表达差异。以siRNA干扰PCRL后,采用qRT-PCR方法检测LNCaP-AI细胞和LNCaP-AD细胞中雄激素受体的表达变化。结果 PCRL在前列腺及前列腺癌组织中特异高表达,LNCaP-AI细胞中PCRL水平高于LNCaP-AD细胞和RWPE-1细胞。干扰PCRL后LNCaP-AI细胞和LNCaP-AD细胞中雄激素受体的表达量上升(前者P<0.000 1)。结论 在前列腺癌中特异高表达的PCRL与前列腺癌的进展有关,PCRL和雄激素受体之间可能存在一定的调控关系。 |
| 关键词: 前列腺肿瘤 长链非编码RNA 雄激素受体 |
| DOI： |
| 投稿时间：2013-06-15修订日期：2013-10-03 |
| 基金项目:国家重点基础研究发展计划(“973”计划, 2012CB518300, 2012CB518306); 国家自然科学基金(81372763). |
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| Expression of prostate cancer related long non-coding RNAs in prostate cancer and its biological function |
| HU Jing jing1△,WANG Fu bo2△,LU Lei1,WANG Lu mei1,LIU Shu peng1,WANG Jia qi1,REN Shan cheng2,XU Gui xia1,LIU Shan rong1,SUN Ying hao2* |
(1. Clinical Experiment Center, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
2. Department of Urology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
△Co-first authors.
*Corresponding author.) |
| Abstract: |
| Objective To investigate the expression of prostate cancer related long non-coding RNAs (PCRL) in prostate cancer and its biological function. Methods The expression levels of PCRL in prostatic cancer tissues, adjacent tissues and tissues of other organs were examined by qRT-PCR. The expression levels of PCRL in androgen-dependent (LNCaP-AD), androgen-independent (LNCaP-AI) prostatic cancer cell lines and normal prostate epithelial cells (RWPE-1) were also compared by using qRT-PCR. Finally, the expression levels of androgen receptor were compared between LNCaP-AD and LNCaP-AI prostatic cancer cells by qRT-PCR after siRNA interference of PCRL expression. Results PCRL were highly expressed in the prostate and prostatic cancer tissues. Expression of PCRL was notably higher in LNCaP-AI cells than in LNCaP-AD and RWPE-1 cells. Interference of PCRL expression in LNCaP-AI and LNCaP-AD cells significantly increased androgen receptor level (P<0.000 1 for LNCaP-AI cells). Conclusion Overexpression of PCRL in prostatic cancer is related to the progression of prostatic cancer; and regulatory relation may exist between PCRL and androgen receptor. |
| Key words: prostatic neoplasms long non-coding RNA androgen receptors |
