| 摘要: |
| 目的 探讨骨髓间充质干细胞对肾癌细胞系A498增殖和侵袭能力的影响。方法 将骨髓间充质干细胞及人成纤维细胞分别与肾癌A498细胞共培养, 利用流式细胞仪测定细胞周期, 检测骨髓间充质干细胞及人成纤维细胞对A498细胞增殖能力的影响;Transwell侵袭实验分析肾癌A498细胞体外侵袭能力;qRT-PCR检测共培养后肾癌A498细胞Snail和E-cadherin基因的表达。结果 骨髓间充质干细胞可明显抑制A498细胞的增殖及侵袭能力(P<0.05);qRT-PCR结果表明, 与骨髓间充质干细胞共培养后, A498细胞中Snail基因表达下调(P<0.05), 而E-cadherin基因表达上调(P<0.05)。成纤维细胞对A498细胞所起的作用与骨髓间充质干细胞相反。结论 骨髓间充质干细胞体外可抑制A498细胞的增殖和侵袭。 |
| 关键词: 肾肿瘤 骨髓 间质干细胞 细胞增殖 肿瘤侵袭 |
| DOI：10.3724/SP.J.1008.2015.00488 |
| 投稿时间：2014-09-11修订日期：2015-01-21 |
| 基金项目:福建省科技厅基金(2012D022). |
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| Inhibitory effect of bone marrow mesenchymal stem cells against proliferation and invasion of human renal cell carcinoma A498 cells |
| DENG Zhen,LIU Jun,ZHU Ling-feng,CHEN Shu-shang,WU Wei-zhen,TAN Jian-ming* |
(Department of Urology, Fuzhou General Hospital, PLA Nanjing Military Area Command, Fuzhou 350001, Fujian, China
*Corresponding author) |
| Abstract: |
| Objective To study the inhibitory effect of bone marrow mesenchymal stem cells (BM-MSCs) on the proliferation and invasion of human renal cell carcinoma cell line A498. Methods Human renal cell carcinoma cell line A498 cells were co-cultured with BM-MSCs or human dermal fibroblasts-adult (HDF-a) cells separately, and then cell proliferation was detected by flow cytometry and cell invasion ability was detected by transwell assay in each group. qRT-PCR was used to exam Snail and E-cadherin mRNA expression in A498 cells, HDF-a-treated A498 cells and BM-MSCs-treated A498 cells. Results BM-MSCs greatly inhibited the proliferation and invasion of A498 cells (P<0.05). qRT-PCR showed that co-culture with BM-MSCs significantly down-regulated Snail mRNA expression (P<0.05) and up-regulated E-cadherin mRNA expression (P<0.05) in A498 cells. Compared with BM-MSCs, HDF-a played an opposite role as for the above items. Conclusion BM-MSCs play an important role in the proliferation and invasion of LNCaP cells, which may cast new light for the biotherapy of human renal cell carcinoma cancer. |
| Key words: kidney neoplasms bone marrow mesenchymal stem cells cell proliferation neoplasm invasiveness |
