| 摘要: |
| 目的 观察电针刺激对中老年部分雄激素缺乏综合征(PADAM)大鼠模型生殖内分泌及肾组织超氧化物歧化酶(SOD)和丙二醛(MDA)的影响。方法 将40只大鼠随机分成2组:正常组(n=10)和模型组(n=30),模型组采用腹腔注射环磷酰胺20 mg/(kg·d)建造PADAM模型。从造模成功的大鼠中随机选取24只平均分为3组:电针组、药物组和对照组,分别以电针刺激、丙酸睾酮和生理盐水治疗8周。分别于造模前、造模后1 d和治疗后1 d观察大鼠一般情况,测定大鼠悬尾时间和强迫游泳时间及血清总睾酮(TT)、游离睾酮(FT)的值。于治疗后测定肾脏中SOD、MDA含量,进行统计分析。结果 采用环磷酰胺造模后,模型组大鼠血清TT、FT水平下降(P<0.01)且均低于正常组血清值99%可信区间,行为学有所改变,类似于PADAM病理变化,表明造模成功。两治疗组血清TT、FT含量较治疗前及对照组升高(P<0.01),悬尾时间和强迫游泳时间改善(P<0.01);肾脏SOD含量高于对照组(P<0.01)而MDA含量低于对照组(P<0.01);两治疗组比较差异无统计学意义(P>0.05)。结论 电针可提高PADAM大鼠血清TT、FT水平,提高其肌张力和抗疲劳能力,并能改善其抑郁状态,其作用与丙酸睾酮相当。电针提高PADAM大鼠体内SOD而降低MDA,提示电针治疗PADAM可能与其改善体内自由基系统有关联。 |
| 关键词: 电针 部分雄激素缺乏综合征 睾酮 超氧化物歧化酶 丙二醛 |
| DOI：10.3724/SP.J.1008.2015.00161 |
| 投稿时间：2014-04-25修订日期：2014-11-01 |
| 基金项目:重庆市教委课题(KJ100321),重庆医科大学基金资助项目(XBYB2008090). |
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| Effect of electro-acupuncture on reproductive endocrine and renal contents of SOD and MDA in aging male rats with partial androgen deficiency |
| REN Yi1,YANG Xiao-guang1,ZHANG Yu2,LI Xue-zhi1* |
(1. College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 400016, China;
2. State Key Laboratory of Ultrasound Engineering in Medical Co-founded by Chongqing and the Ministry of Science and Technology, College of Biomedical Engineering, Chongqing Medical University, Chongqing 400016, China
*Corresponding author) |
| Abstract: |
| Objective To observe the effect of electro-acupuncture on reproductive endocrine and renal contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in aging male (PADAM) rats with partial androgen deficiency. Methods Forty male rats were randomly divided into normal control group (n=10) and model group (n=30). Rats in the model group were injected with cyclophosphamide 20 mg/(kg·d) into the abdominal cavities to establish PADAM model. The 24 successful model rats were further randomized into electro-acupuncture, androlin treatment and blank groups; rats received corresponding treatment for 8 weeks. The general conditions of the animals were observed before and 1 day after modeling and at the end of treatment. Tail suspension experiments and exhaustion swimming tests were performed; and the serum total testosterone (TT) and free testosterone (FT) levels were determined. The contents of SOD and MDA in renal tissues were examined at the end of the experiments by hydroxylamine method. Results After treatment with cyclophosphamide, the serum levels of TT and FT in rats were significantly decreased (P<0.01), being lower than the 99% confidence interval of the normal control group, and the animals developed behaviors similar to those of PADAM, indicating successful modeling. The serum TT and FT levels in electro-acupuncture group and androlin group were significantly higher than those in the pre-therapy and blank control groups (P<0.01); the duration of immobility and exhaustion swimming were also significantly improved in the two treatment groups (P<0.01); and SOD activities in the kidney were significantly increased (P<0.01) and MDA contents were significantly decreased (P<0.01) in electro-acupuncture group and androlin group; while no significant differences were found between electro-acupuncture group and androlin group (P>0.05). Conclusion Electro-acupuncture can increase serum TT and FT levels in PADAM rats, improve the depression state, myodynamia and energy in the aging rats, with its effect being equivalent to that of testosterone propionate. Electro-acupuncture can increase SOD level and decease MDA level in PADAM rats, indicating that electro-acupuncture may function by improving the free radicals system of PADAM rats. |
| Key words: electroacupuncture partial androgen deficiency of aging male testosterone saperoxide dismutase malondialdehyde |
