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| 原发性肝癌患者甲胎蛋白基因多态性与其在外周血中表达水平的相关性 |
| 吉强,顾星,周军,殷月鹏,陈冬梅,陈思佳,董郭平,高春芳* |
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(第二军医大学东方肝胆外科医院实验诊断科, 上海 200438
*通信作者) |
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| 摘要: |
| 目的 探讨原发性肝细胞癌（hepatocellular carcinoma，HCC）患者甲胎蛋白（α-fetoprotein，AFP）基因多态性与外周血AFP水平的相关性。 方法 采用聚合酶链反应和连接酶检测反应法（PCR-LDR）检测我院收治的335例HCC患者AFP基因多个位点多态性并检测AFP血清水平，运用SPSS 13.0软件对结果进行统计学分析。 结果 AFP基因-205 C>G位点GG基因型分布在AFP水平≥20和<20 ng/mL两组间差异有统计学意义（GG vs CC：χ2=4.389，P=0.036），G等位基因分布在AFP水平≥400和<400 ng/mL两组间差异有统计学意义（G vs C：χ2=4.410，P=0.036）。AFP基因intron1 A>T、intron7 A>G、3’UTR A>G和intron2 A>G基因多态性与AFP基因表达水平无密切相关性。 结论 在中国汉族人群HCC患者中，AFP基因-205 C>G位点C等位基因及CC基因型携带者外周血AFP水平明显升高。 |
| 关键词: 甲胎蛋白类 单核苷酸多态性 肝细胞癌 乙型肝炎 |
| DOI：10.3724/SP.J.1008.2014.00603 |
| 投稿时间：2014-04-29修订日期：2014-05-23 |
| 基金项目:国家科技重大专项项目（2012ZX10002-016）. |
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| Relationship between α-fetoprotein polymorphisms and peripheral α-fetoprotein level in hepatocellular carcinoma patients |
| JI Qiang,GU Xing,ZHOU Jun,YIN Yue-peng,CHEN Dong-mei,CHEN Si-jia,DONG Guo-ping,GAO Chun-fang* |
(Department of Laboratory Medicine, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China
*Corresponding authors.) |
| Abstract: |
| Objective To study the relationship between α-fetoprotein (AFP) polymorphisms and peripheral AFP level in hepatocellular carcinoma (HCC) patients. Methods Using polymerase chain reaction-ligation detection reaction (PCR-LDR) technique, we determined the polymorphisms of AFP of 335 HCC patients; the serum levels of AFP were also determined. SPSS 13.0 software was used for statistical analysis. Results The GG genotype frequency of -205 C>G of AFP gene was significantly different between patients with AFP values ≥20 and <20 ng/mL (GG vs CC: χ2= 4.389, P=0.036). The frequency of G allele was also found significantly different between patients with AFP values ≥400 and <400 ng/mL (G vs C: χ2=4.410, P=0.036). No relationship was found between AFP expression and other polymorphisms, including intron1 A>T, intron7 A>G, 3'UTR A>G and intron2 A>G, in AFP gene. Conclusion In HCC patients among Chinese Han population, the C allele and CC genotype of -205 C>G polymorphism site in AFP gene is associated with higher AFP values. |
| Key words: alpha-different fetoproteins single nucleotide polymorphism hepatocellular carcinoma hepatitis B |
