| 摘要: |
| 目的 研究藳本内酯(ligustilide, LIG)对低灌注大鼠大脑皮质的神经保护作用,并探寻其可能的机制。方法 通过结扎SD大鼠双侧颈总动脉(2VO)制备低灌注模型。假手术组不结扎。术后第8天,部分模型组大鼠灌胃给予LIG治疗(剂量为80 mg/kg),1次/d,连续21 d。停药7 d后取大鼠大脑,进行冰冻切片、尼氏染色、免疫组化和免疫荧光等实验,检测大脑皮质区域神经元尼氏小体、神经元特异性核蛋白(NeuN)、微管相关蛋白2(MAP-2)和Caspase-3的表达变化。结果 LIG治疗组大鼠皮质区尼氏小体和NeuN阳性神经元数量较模型组增加(P<0.01); LIG治疗组大鼠皮质区Caspase-3阳性细胞数量较模型组减少(P<0.01)。LIG治疗组大鼠皮质区MAP-2阳性结构较模型组得到改善。结论 LIG可能通过减少低灌注大鼠皮质区神经元的凋亡和调节MAP-2的表达发挥神经保护作用。 |
| 关键词: 藁本内酯 慢性低灌注 神经保护 细胞凋亡 |
| DOI：10.16781/j.0258-879x.2016.02.0251 |
| 投稿时间：2015-05-24修订日期：2015-12-20 |
| 基金项目:江苏省科技厅高技术研究计划项目(BG2007607), 南通大学校级自然科学类科研基金(12Z020). |
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| Neuron protective effects of ligustilide on cortex region of cerebral hypoperfusion rats |
| WANG Cheng-niu1,FENG Zhan-bo2,PENG Bin2,ZHU Li2* |
(1. Basic Medical Research Center, Medical School, Nantong University, Nantong 226001, Jiangsu, China;
2. Institute for Nautical Medicine, Nantong University, Nantong 226001, Jiangsu, China
*Corresponding author) |
| Abstract: |
| Objective To investigate the neuron protective effects of ligustilide (LIG) on cortex regions of cerebral hypoperfusion rats and the possible mechanisms of the protective effect. Methods Bilateral common carotid arteries (two-vessel occlusion, 2VO) of Sprague-Dawley rats were occluded to induce the chronic cerebral hypoperfusion models. The sham-operated controls (Sham group) were not occluded. The model rats were treated with LIG (80 mg/kg, by oral) once a day from the 8th day after surgery for 21 d. The rats were sacrificed 7 d after stopping LIG treatment, and the brain tissues of rats were made into frozen sections. Nissl staining, immunohistochemistry and immunofluorescence experiments were performed. Coronal sections of the cortex were stained with cresyl violet or with antibodies for neuronal specific nuclear protein (NeuN), microtubule-associated protein-2 (MAP-2) and cysteinyl aspartate specific proteinase 3 (Caspase-3). Results The number of Nissl body and NeuN-positive cells were increased significantly in the cortex region of LIG treatment group compared with those in the model group (P<0.01). The number of Caspase-3 positive cells in rat cortex region was decreased significantly in LIG treatment group compared with that in the model group (P<0.01). LIG treatment also improved the MAP-2 positive structures in the cortex region compared with the model group. Conclusion LIG may have neuron protective effects on cortex regions of cerebral hypoperfusion rats through reducing apoptosis of hypoperfusion rat cortical neurons and regulating the expression of MAP2. |
| Key words: ligustilide chronic cerebral hypoperfusion neuroprotective apoptosis |
