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| 壬基酚对大鼠5-羟色胺及其2A受体的影响 |
| 杨童旺1,赖玉婷1,郭佑廷1,欧阳俊彦1,林媛1,吕宝怡1,柳春红1,2* |
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(1. 华南农业大学食品学院食品科学系, 广州 510642;
2. 广东省食品质量安全重点实验室, 广州 510642
*通信作者) |
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| 摘要: |
| 目的 观察壬基酚(NP)对大鼠血浆和尿液中5-羟色胺(5-hydroxy tryptamine, 5-HT)及血小板中5-HT和5-HT2A受体含量的影响, 探讨壬基酚对大鼠5-HT和5-HT2A受体影响的毒效应机制。方法 将24只SD雄性大鼠分为阴性对照组和NP低、中、高剂量组[30、90、270 mg/(kg·d)], 隔日灌胃染毒28 d后检测大鼠血浆中5-HT、血小板中5-HT和5-HT2A受体含量, 并检测灌胃后收集到的24 h尿液中5-HT的含量。结果 染毒28 d后, 随NP暴露的剂量增加, 各组大鼠血浆、血小板及尿液中5-HT含量升高, 血小板中5-HT2A受体表达则下降。NP暴露中、高剂量组大鼠血浆及血小板中5-HT含量均高于对照组(P<0.01;P<0.01), NP暴露高剂量组大鼠血小板5-HT2A受体表达低于对照组(P<0.01)。第4~28天, NP暴露低、中、高剂量组大鼠的尿液5-HT含量均高于对照组(P<0.01)。结论 NP暴露剂量与大鼠血小板5-HT、5-HT2A受体及血浆和尿液中5-HT含量均呈剂量-效应关系, 提示NP通过影响大鼠5-HT水平和5-HT2A受体表达而产生毒效应。 |
| 关键词: 壬基酚 5-羟色胺 5-HT2A受体 毒效应 |
| DOI：10.3724/SP.J.1008.2014.01358 |
| 投稿时间：2014-06-12修订日期：2014-09-23 |
| 基金项目:广东省自然科学基金(S2011010001045);广东省科技计划项目(2012B091400001, 2012B090600005). |
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| Effects of nonylphenol on 5-hydroxytryptamine and its 2A receptor in rats |
| YANG Tong-wang1,LAI Yu-ting1,GUO You-ting1,OUYANG Jun-yan1,LIN Yuan1,LÜ Bao-yi1,LIU Chun-hong1,2* |
(1. Department of Food Science, Food College, South China Agricultural University, Guangzhou 510624, Guangdong, China;
2. Guangdong Provincial Key Laboratory of Food Quality and Safety, Guangzhou 510642, Guangdong, China
*Corresponding author) |
| Abstract: |
| Objective To observe the effect of nonylphenol (NP) on plasma and urine 5-hydroxytryptamine (5-HT) levels and expression of 5-HT and of 5-HT2A receptor in the platelets of rats, so as to explore the toxic mechanisms of NP on 5-HT and 5-HT2A receptor. Methods A total of 24 male SD rats were equally randomized into 4 groups: solvent control group, NP low-dose group (30 mg/[kg·d]), NP medium-dose group (90 mg/[kg·d]) and NP high-dose group (270 mg/[kg·d]). The animals were gavaged with NP every other day for 28 days; then the plasma and 24 h urine levels of 5-HT, 5-HT and 5-HT2A expression in the platelets were examined. Results The levels of 5-HT in the plasma and platelets of rats were increased and the expression of 5-HT2A receptor in platelets was decreased with the increase of NP exposure dose. The plasma and platelet 5-HT levels in the medium- and high-NP dose groups were significantly higher than those in the control group(P<0.01); the platelet level of 5-HT2A receptor in the high-NP dose group was significantly lower than that in the control group (P<0.01). The three NP dose groups had significantly higher urine 5-HT levels compared with the control group during 4-28 days of NP exposure(P<0.01). Conclusion There are dose-dependent effects between NP exposure dose and plasma, urine 5-HT levels and platelet 5-HT, 5-HT2A receptor levels in rats, suggesting that NP might exert its toxic effect by affecting 5-HT level and 5-HT2A receptor expression. |
| Key words: nonylphenol 5-hydroxytryptamine 5-HT2A receptor toxic effect |
