| 摘要: |
| 目的 探讨2型糖尿病(T2DM)周围神经病变(diabetic peripheral neuropathy, DPN)患者外周血中CD4+CD25+Foxp3+调节性T细胞(Treg)的变化及其检测的临床应用。方法 采用流式细胞技术多色荧光分析法,对20例初诊老年2型DPN患者(DPN组)、20例初诊老年T2DM不伴并发症患者(T2DM组)及20例性别、年龄匹配的健康对照者(CON组)外周血中Treg细胞进行测定,对各组结果进行统计分析并进行比较。结果 与CON组相比,DPN组和T2DM组患者外周血CD4+CD25+Foxp3+ Treg细胞数量均减少(P<0.05),且DPN组少于T2DM组患者(P<0.05)。DPN组患者外周血CD4+CD25+Foxp3+ Treg细胞比例与密歇根神经病变筛查量表(Michigan neuropathy screening instrument, MNSI)评分呈负相关,但无统计学意义;与密歇根糖尿病神经病变评分量表(Michigan diabetic neuropathy score, MDNS)评分负相关,有统计学意义(r=-0.339,P<0.05)。结论 老年DPN患者外周血CD4+CD25+Foxp3+ Treg细胞数量减少,可能在DPN的发生、发展中起到一定作用。 |
| 关键词: 2型糖尿病 糖尿病神经病变 调节性T淋巴细胞 |
| DOI：10.3724/SP.J.1008.2015.01007 |
| 投稿时间：2015-05-17修订日期：2015-08-30 |
| 基金项目:国家自然科学基金(81300672,81100585). |
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| Detection and clinical significance of regulatory T cells in peripheral blood of elderly patients with type 2 diabetic peripheral neuropathy |
| YANG Xue1Δ,TANG Wei2Δ,SHI Yong-quan2* |
(1. The Fourth Department of Cadre Ward, General Hospital, PLA Jinan Military Area Command, Jinan 250031, Shandong, China;
2. Department of Endocrinology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
△Co-first authors
* Corresponding author) |
| Abstract: |
| Objective To investigate the changes of CD4+CD25+Foxp3+ regulatory T cell (Treg) in the peripheral blood of elderly patients with type 2 diabetic peripheral neuropathy (DPN) and its clinical implication. Methods Treg cells were detected by flow cytometry in the peripheral blood of elderly patients with type 2 DPN (DNP group, 20 cases), type 2 diabetes mellitus (T2DM) with no complications (T2DM group, 20 cases) and age-matched healthy volunteers (CON group, 20 cases). The results were statistically analyzed and compared between different groups. Results The numbers of CD4+CD25+Foxp3+ Treg in DPN group and T2DM group were significantly less than that in the healthy volunteers (P<0.05), and the number of Treg cells in DPN group was significantly less than that in T2DM group (P<0.05). The proportion of CD4+CD25+Foxp3+ Treg was negatively associated with Michigan neuropathy screening instrument (MNSI) scores in DPN group, but with no statistical significance; however, the proportion had a significantly negative correlation with Michigan diabetic neuropathy score (r=-0.339, P<0.05). Conclusion The number of CD4+CD25+Foxp3+ Treg is decreased in the peripheral blood of elderly patients with DPN, which may play a role in the development and progression of DPN in elderly patients. |
| Key words: type 2 diabetes mellitus diabetic neuropathies regulatory T-lymphocytes |
