| 摘要: |
| 目的 本文主要探讨血清microRNA(miR)-21/155/181a/18b在具有失代偿性心力衰竭症状的扩张型心肌病(扩心病)患者中的表达水平。 方法 收集40例扩心病患者和30名健康对照者, 通过实时免疫荧光定量PCR 反应检测血清miRNA。分析miR-21等miRNA和其他临床指标如N末端脑钠肽前体(NT-proBNP)、超敏C-反应蛋白(hsCRP)、左室射血分数( LVEF%)、左室舒张末期内径(LVEDD)之间的相关性。 结果 血清miR-21在扩心病患者中的相对表达量低于健康对照组(P<0.01)。在所有的试验者中, 血清miR-21与LVEF%呈正相关, 与LVEDD呈负相关(P<0.001)。 结论 与健康对照者相比, 血清心肌重构相关的miR-21在失代偿性心力衰竭的扩心病患者中表达下调, 并与疾病的严重程度相关。 |
| 关键词: 微RNAs 扩张型心肌病 血清 诊断 |
| DOI：10.16781/j.0258-879x.2016.03.0379 |
| 投稿时间：2015-05-30修订日期：2015-11-07 |
| 基金项目:国家"863"高技术研究发展计划(2007AA022450), 国家自然科学基金(30870622). |
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| Serum microRNA-21 expression is significantly down-regulated in patients with dilated cardiomyopathy |
| XU Gui-fen1,WENG Zhi-yuan1,YANG Wei2* |
(1. Department of Cardiology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, Fujian, China;
2. Department of Cardiology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China
*Corresponding author) |
| Abstract: |
| Objective To address the levels of serum miR-21/155/181a/18b in dilated cardiomyopathy (DCM) patients at a progressive heart failure stage. Methods The serum miRNAs were measured by real-time reverse-transcription PCR in 40 DCM patients and in 30 healthy controls. Pearson's correlation coefficient was used to calculate the potential correlation between miRNAs and other indicators, such as human N-terminal pro-brain natriuretic peptide (NT-proBNP), high-sensitivity C-reactive protein (hsCRP), left ventricular ejection fraction (LVEF%), and left ventricular end-diastolic diameter (LVEDD). Results Our findings revealed that serum miR-21 were significantly reduced in DCM patients compared with the healthy controls(P<0.01). We also found that serum miR-21 was positively correlated with LVEF% and negatively correlated with LVEDD (P<0.001). Conclusion The serum level of cardiac remodeling-associated miR-21 is decreased in DCM patients at a progressive heart failure stage compared to the healthy controls, which may be also related to the disease severity. |
| Key words: microRNAs dilated cardiomyopathy serum diagnosis |
