| 摘要: |
| 目的 观察人主动脉夹层组织中Myc associated factor X(MAX)的表达情况,并探讨其相关生物学功能。方法 采用荧光定量RT-PCR和蛋白质印迹检测MAX在15例主动脉夹层组织中的表达情况。通过腺病毒载体转染人主动脉平滑肌细胞使其过表达MAX,采用Cell Counting Kit-8(CCK-8)、流式细胞仪等技术明确过表达MAX对主动脉平滑肌细胞增殖、凋亡的影响。结果 夹层主动脉组织中MAX的mRNA和蛋白表达水平均高于正常主动脉组织。过表达MAX可以抑制主动脉平滑肌细胞增殖,并促进其凋亡(P<0.05)。结论 MAX可能通过调控主动脉平滑肌细胞增殖、凋亡诱导其丢失,进而参与主动脉夹层的发生、发展。 |
| 关键词: 主动脉瘤 夹层动脉瘤 Myc基因相关X因子 人主动脉平滑肌细胞 细胞增殖 细胞凋亡 |
| DOI：10.3724/SP.J.1008.2015.00943 |
| 投稿时间：2015-06-05修订日期：2015-07-22 |
| 基金项目:国家自然科学基金(81300233). |
|
| Expression and biological functions of Myc associated factor X in human aortic dissection tissue |
| TAN Meng-wei,LIAO Wei-lin,TANG Hao,LU Fang-lin,HAN Lin,XU Zhi-yun* |
(Department of Thoracic and Cardiovascular Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
*Corresponding author) |
| Abstract: |
| Objective To observe the expression of Myc associated factor X (MAX) in aortic dissection tissue, and to discuss its biological functions. Methods MAX expression level was evaluated by qRT-PCR and Western blotting analysis in 15 dissected aorta samples. The adenovirus vector was used to transfect human aortic smooth muscle cells (HASMCs) for overexpression of MAX. The effects of MAX overexpression on proliferation and apoptosis of HASMCs were analyzed by Cell Counting Kit-8 and flow cytometry, respectively. Results MAX mRNA and protein expression levels were significantly higher in the aortic dissection tissue compared with that in the healthy controls. Overexpression of MAX significantly inhibited the proliferation of HASMCs and promoted its apoptosis (P<0.05). Conclusion MAX might induce the loss of HASMCs via regulating their proliferation and apoptosis process, thus play an important role in the development and progression of aortic dissection. |
| Key words: aortic aneurysm dissecting aneurysm Myc associated factor X human aortic smooth muscle cells cell proliferation apoptosis |
