| 摘要: |
| 目的 探讨甾类激素对原代培养的大鼠附睾头部上皮细胞中Bin1b表达的影响。 方法 不同浓度(10-9,10-8,10-7mol/L)的二氢睾酮(dihydrotestosterone, DHT)、(10-9, 10-8mol/L)雌二醇(estradiol, E2)及(10-8,10-7mol/L)地塞米松(dexamethasone, Dex)处理原代培养的附睾头部上皮细胞0~5 d,分别采用1.2％琼脂糖凝胶电泳和PCR的方法检测Bin1b mRNA的表达。 结果 原代培养的附睾上皮细胞中Bin1b mRNA的表达呈时间依赖性减少,培养第2 天Bin1b mRNA水平下调约40％,第3 天下调约70%,5 d时附睾上皮细胞中几乎检测不到Bin1b mRNA的表达。不同浓度的DHT对Bin1b mRNA表达的下降有不同程度的减缓,10-9mol/L的DHT使Bin1b mRNA表达较对照细胞增加约37％(P<0.05),10-8mol/L及10-7mol/L DHT处理后Bin1b mRNA表达进一步的增加。不同浓度的E2和Dex对Bin1b mRNA表达的下降无明显调节作用。 结论 雄激素能上调原代培养的附睾头部上皮细胞中Bin1b的表达,而雌激素和糖皮质激素没有明显的调节作用。 |
| 关键词: 附睾 β防御素 Bin1b 性腺甾类激素 |
| DOI：10.16781/j.0258-879x.2016.01.0022 |
| 投稿时间：2015-06-18修订日期：2015-09-06 |
| 基金项目: |
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| Influence of steroid hormone on expression of Bin1b in primary cultured epididymal epithelial cells |
| SU Jie,LU Jian,HUANG Gao-xiang,CAO Dong-mei* |
(Department of Pathophysiology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China
*Corresponding author.) |
| Abstract: |
| Objective To study the expression of Bin1b in primary cultured epididymal epithelial cells treated with different steroid hormones at various concentrations. Methods Expression of Bin1b mRNA was examined by agarose gel electrophoresis and PCR in primary cultured epididymal epithelial cells, which were treated with dihydrotestosterone (DHT, 10-9,10-8 and 10-7mol/L), estradiol (E2, 10-9 and 10-8mol/L)) or dexamethasone (Dex, 10-8 and 10-7mol/L) at different concentrations. Results The expression of Bin1b mRNA in primary cultured epididymal epithelial cells was decreased in a time-dependent manner. The mRNA level of Bin1b was decreased by 40% at day 2 and 70% at day 3; and it was hardly detectable at day 5. DHT up-regulated the expression of Bin1b mRNA in a dosage-dependent manner compared with corresponding vehicle control. 10-9mol/L DHT up-regulated Bin1b mRNA level by 37% compared with corresponding vehicle control(P<0.05), and it was further up-regulated after the treatment with 10-8mol/L and 10-7mol/L DHT. E2 and Dex at various concentrations showed no significant effects on the expression of Bin1b mRNA in primary cultured epididymal epithelial cells. Conclusion Dihydrotestosterone can up-regulate the expression of Bin1b mRNA in primary cultured epididymal epithelial cells, while estradio and dexamethasone can not. |
| Key words: epididymis β-defensins Bin1b gonadal steroid hormone |
