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| 驻极体外电场对季铵化壳聚糖生长因子纳米粒制备的影响 |
| 郭鑫1△,石岩2△,梁媛媛1,黄平1,崔黎丽3,江键1* |
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(1. 第二军医大学基础部数理教研室, 上海 200433;
2. 第二军医大学海军医学系2012 级学员, 上海 200433;
3. 第二军医大学药学院无机化学教研室, 上海 200433
△共同第一作者
*通信作者) |
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| 摘要: |
| 目的 研究驻极体外电场对季铵化壳聚糖生长因子纳米粒制备及表征的影响，探究季铵化壳聚糖生长因子纳米粒的经皮载药转运机制。方法 以表皮生长因子为模型药物、季铵化壳聚糖为材料、三聚磷酸钠为交联剂，在不同表面电位驻极体产生的外静电场作用下，采用离子交联法制备不同电场条件下的季铵化壳聚糖生长因子纳米粒。透射电镜观察纳米粒的表面形态，粒径-zeta电位仪测定纳米粒的粒径和zeta电位。结果 （1）用离子交联法制备的季铵化壳聚糖生长因子纳米粒呈球形，分布均匀、分散性较好，且具有良好的稳定性。（2）季铵化壳聚糖生长因子纳米粒的粒径随季铵化壳聚糖浓度的增加而增大，季铵化壳聚糖的浓度可能是调控季铵化壳聚糖生长因子纳米粒粒径的主要因素。（3）外静电场能引起壳聚糖生长因子纳米粒极化。季铵化壳聚糖生长因子纳米粒的粒径随静电场场强的增大而减小，zeta电位的变化也与外加电场的极性密切相关。（4）经静电场处理后的季铵化壳聚糖生长因子纳米粒的粒径随存放时间的推移而减小。结论 季铵化壳聚糖生长因子纳米粒的形态、粒径和zeta电位与外加电场的场强大小和极性密切相关，要制备粒径适当、稳定性好的纳米粒，必须合理选择电场参数。 |
| 关键词: 驻极体 静电场 表皮生长因子 纳米粒子 壳聚糖 |
| DOI：10.16781/j.0258-879x.2016.07.0805 |
| 投稿时间：2016-03-06修订日期：2016-04-28 |
| 基金项目:国家自然科学基金（51477175），第二军医大学校级军事课题（2013JSD2），第二军医大学大学生创新能力培养基金（PH2014003）. |
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| Effect of electret electrostatic-field on preparing nanopaticles carrying quaternary ammonium chitosan and epidermal growth factor |
| GUO Xin1△,SHI Yan2△,LIANG Yuan-yuan1,HUANG Ping1,CUI Li-li3,JIANG Jian1* |
(1. Department of Physics and Mathematics, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China;
2. Student of Grade 2012, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China;
3. Department of Inorganic Chemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
* Corresponding author) |
| Abstract: |
| Objective To study the effect of electret electrostatic-field on the preparation and characteristics of nanopaticles carrying quaternary ammonium chitosan and epidermal growth factor (EGF), so as to explore the percutaneous transport mechanism of drug-loading nanoparticles. Methods Under different electret electrostatic-fields, ionic crossing method was used to prepare EGF-loading chitosan nanoparticles using EGF as model drug, chitosan as material, and sodium tripolyphosphate(TPP) as crosslinking agent. Transmission electron microscope was employed to observe the surface morphology of nanoparticles; the particle size and the zeta potential of the EGF-loading nanoparticles were also examined. Results (1) The EGF-loading chitosan nanoparticles prepared in this study had uniform distribution, good dispersibility and stability. (2) The size of the EGF-loading nanoparticles increased with the increase of chitosan concentration, indicating that chitosan concentration might be the main reason for the nanoparticle size. (3) The particle size of the EGF-loading chitosan nanoparticles decreased with the increase of electrostatic field; and the change of the zeta potential was closely related to the polarity of the applied electric field. (4) The particle size of EGF-loading nanoparticles decreased with the increase of storage time. Conclusion The morphology, particle size and zeta potential of chitosan nanoparticles are closely related to the intensity and polarity of the applied electric field; proper electric field parameters should be chosen to prepare high quality chitosan nanoparticles with appropriate particle size and stability. |
| Key words: electret electrostatic field epidermal growth factor nanoparticles chitosan |
