| 摘要: |
| 目的 盐酸左氧氟沙星眼用即型凝胶治疗兔金黄色葡萄球菌角膜炎的药效学研究。方法 选取60只双眼均正常的新西兰大白兔，给予左眼角膜基质内注射10 μL含105个菌落形成单位（colony-forming unit，CFU）的金黄色葡萄球菌菌液，建立角膜炎动物模型。挑选炎症程度相近的48只兔，采用完全随机设计方法分为4组，即24 h基线组、磷酸盐缓冲液（PBS）空白对照组、盐酸左氧氟沙星滴眼液组及盐酸左氧氟沙星眼用即型凝胶组，每组12只。观察24 h基线组兔眼临床炎症情况并评分，观察角膜病理变化及进行细菌菌落计数；其余3组每日治疗3次，至第7天最后一次给药后2 h对3组兔的处理同24 h基线组。各组角膜临床炎症评分和角膜细菌菌落计数值比较均使用多个样本均数比较中的完全随机设计资料的方差分析，即F检验。结果 第7天时，相对于PBS空白对照组，盐酸左氧氟沙星滴眼液与盐酸左氧氟沙星眼用即型凝胶均降低角膜炎感染的临床症状（P=0.00），角膜炎临床评分分别为4.21±1.10和3.63±0.86；第7天时，盐酸左氧氟沙星滴眼液组与盐酸左氧氟沙星眼用即型凝胶组兔眼角膜菌落数均降低（P=0.00），角膜菌落计数分别为（4.87±0.05）和（4.64±0.10）CFU，差异有统计学意义（P=0.00）。结论 盐酸左氧氟沙星眼用即型凝胶局部给药治疗金黄色葡萄球菌角膜炎可明显改善临床症状、降低角膜菌落数，其作用优于常用的盐酸左氧氟沙星滴眼液，是一种有效的新型眼用制剂。 |
| 关键词: 盐酸左氧氟沙星 眼用即型凝胶 角膜炎 金黄色葡萄球菌 |
| DOI：10.16781/j.0258-879x.2016.09.1070 |
| 投稿时间：2015-12-21修订日期：2016-06-20 |
| 基金项目:国家科技重大专项（2011ZXJ09104-10C）. |
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| Efficacy of levofloxacin hydrochloride eye gel in treatment of Staphylococcus aureus keratitis in rabbits |
| LIU Fu-ping,GU Cao,SUN Wei-feng,QIN Hai-feng,ZHANG Yue-lu,ZHAO Shi-hong* |
(Department of Ophthalmology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
*Corresponding author) |
| Abstract: |
| Objective To observe the efficacy of levofloxacin hydrochloride eye gel (LHEG) in treatment of Staphylococcus aureus keratitis in rabbits. Methods Sixty rabbits with normal eyes were used in this study; their left eyes received intrastromal injection of 10 μL bacterial suspension containing 105 colony-forming unit (CFU) Staphylococcus aureus to establish Staphylococcus aureus keratitis model. Forty-eight rabbits with similar severity of inflammation were randomly divided into four groups:24 h baseline group, phosphate buffer solution (PBS) control group, levofloxacin hydrochloride eye drop (LHED) group, and LHEG group. Rabbits in the 24 h baseline group received slit-lamp examination and were given clinical score, and their corneas were collected for histopathology examination and bacterial quantitation. The other three groups received treatment with PBS, LHED or LHEG three times a day for seven successive days. In the last day, rabbits in these three groups received the same examination as that in the 24 h baseline group. The clinical scores and cornea bacterial quantitation were analyzed with variance analysis, and a P value less than 0.05 was considered as statistically significant. Results Compared with PBS control group, clinical symptoms of LHED group and LHEG group were significantly reduced(P=0.00) on the seventh day, with the clinical scores being (4.21±1.10) and (3.63±0.86), respectively; the numbers of bacterial colonies were also significantly reduced in LHED group and LHEG group (P=0.00), with the numbers being (4.87±0.05) and (4.64±0.10) CFU, respectively. Moreover, LHEG group had a significantly better bacteriostatic effect than LHEG group (P=0.00). Conclusion Local application of LHEG can greatly inhibit the growth of bacterial colony of Staphylococcus aureus and improve the clinical signs of keratitis, with the efficacy being better than that of routinely used LHED in rabbit model of Staphylococcus aureus keratitis. |
| Key words: levofloxacin hydrochloride in situ forming eye gel keratitis Staphylococcus aureus |
