| 摘要: |
| 目的 建立白狼毒药材中月腺大戟素A的含量测定方法。方法 采用高效液相色谱法,色谱柱为Agilent Zorbax SB-C18 (4.6 mm×150 mm,5 μm),流动相为乙腈:0.1%甲酸水溶液=55:45,等度洗脱,流速1.0 mL·min-1,检测波长为290 nm,柱温25℃,进样量20 μL,运行时间为20 min。结果 月腺大戟素A与周围干扰峰达到基线分离,线性范围为4.545~227.3 μg·mL-1,相关系数r=0.9999;日内、日间精密度均小于2%;平均回收率为(99.1±3.4)%(n=6),狼毒大戟2个批次药材及月腺大戟对照药材中月腺大戟素A含量分别为(56.73±1.09)、(18.98±2.11)及(235.2±2.4) μg/g(n=6)。结论 该法简便快捷、测定结果准确,重复性好,可用于白狼毒药材中月腺大戟素A的含量测定。 |
| 关键词: 白狼毒 月腺大戟 狼毒大戟 月腺大戟素A 高压液相色谱法 |
| DOI：10.16781/j.0258-879x.2016.04.0517 |
| 投稿时间：2016-02-04修订日期：2016-03-28 |
| 基金项目:国家自然科学基金青年项目(81303300). |
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| An HPLC method for determination of ebracteolatain A content in the root of Euphorbia fischeriana Steud and Euphorbia ebracteolata Hayata |
| LI Yun-qing,ZHAO Liang,WANG Xin-xia,QIAN Xian,LI Sheng-jian,ZHANG Feng,LÜ Lei,ZHANG Guo-qing* |
(Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China
*Corresponding author) |
| Abstract: |
| Objective To establish a method for determination of ebracteolatain A content in the root of traditional Chinese medicine white Langdu, including Euphorbia fischeriana Steud and Euphorbia ebracteolata Hayata. Methods An HPLC-DAD method was created for determination at the following condition:the column was Agilent Zorbax SB-C18 (4.6 mm×150 mm, 5 μm);mobile phase was acetonitrile:0.1% formic acid in water=55:45(V:V), isocratic elution, the flow rate was 1.0 mL·min-1, the temperature of column was 25℃, the detection wavelength was set at 290 nm, the injection volume was 20 μL, and the running time was 20 min. Results Ebracteolatain A was separated from the interference in the baseline, and the linear range was 4.545-227.3 μg·mL-1, with the linear correlation being 0.9999 for ebracteolatain A. The result of intra-day and inter-day precisions were both within 2%(n=3), and the average recovery was (99.14±3.4)%(n=6). The content of ebracteolatain A in two batches of Euphorbia fischeriana Steud and Euphorbia ebracteolata Hayata were (56.73±1.09) μg/g, (18.98±2.11) μg/g and (235.2±2.4) μg/g (n=6), respectively. Conclusion The present method is simple, rapid, accurate and convenient for determination of ebracteolatain A in the root of traditional Chinese medicine white Langdu. |
| Key words: white Langdu Euphorbia fischeriana Steud Euphorbia ebracteolata Hayata ebracteolatain A high pressure liquid chromatography |
