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| 一种新型巴沙鱼皮胶原支架材料的理化性能及体外降解性 |
| 陈彤1△,夏凯2△,吴刚3,祖丽皮也·阿不力克木1,朱庆丰1,王少海1*,易杨华4* |
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(1. 第二军医大学长海医院口腔科, 上海 200433;
2. 上海海洋大学食品学院海洋生物制药研究室, 上海 201306;
3. 解放军85医院口腔科, 上海 200052;
4. 第二军医大学药学院海洋药物研究中心, 上海 200433
△共同第一作者
*通信作者) |
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| 摘要: |
| 目的 以巴沙鱼皮为原料制备新型胶原支架材料，并对该材料的结构特性、物理性质以及体外降解性进行检测和分析，以探索巴沙鱼是否可以取代陆生哺乳动物作为胶原支架材料的合适来源。方法 巴沙鱼皮经过反复脱脂、脱色、脱杂处理后，冷冻干燥成膜状。采用凯氏定氮法测定粗蛋白含量，扫描电镜观察和分析材料的结构以及孔隙的大小和分布，液体置换法测孔隙率，万能试验机检测抗拉强度。观察材料的黏度随温度的变化情况，以检测材料的变性温度。将材料浸泡在置于37℃恒温震荡箱的磷酸盐缓冲液（0.1 mol/L，pH 7.4）中，检测材料的吸水率和失重率。结果 材料的粗蛋白含量为95.2%。肉眼观察厚薄均匀。扫描电镜显示材料的一面较为粗糙，可见多孔结构，孔径大小不一，分布均匀；另一面光滑，孔隙致密。孔隙率（55.50±1.94）%，厚度（0.66±0.10） mm，抗拉强度（18.82±0.94） MPa。未经交联的材料变性温度为34℃，经热交联后为36℃。48 h吸水率（379.77±77.81）%。经热交联后的材料28 d的失重率为（80.22±2.49）%，缓冲液pH为6.67±0.05。结论 巴沙鱼皮胶原支架材料可制成厚薄均匀、具有致密层和疏松层双层不同结构的生物膜。材料具有良好的机械强度、较为适宜的变性温度，但降解速率较快，有待改进。 |
| 关键词: 巴沙鱼 胶原 组织支架 体外降解 可降解聚合物 理化性能 组织工程 |
| DOI：10.16781/j.0258-879x.2017.01.0041 |
| 投稿时间：2016-11-30修订日期：2016-12-19 |
| 基金项目:国家海洋局海洋公益性行业科研专项（201405015，201305013），国家自然科学基金（81271177）. |
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| Physicochemical properties and in vitro degradation of a novel collagen scaffold material from Basa fish (Pangasisus haniltoa) skin |
| CHEN Tong1△,XIA Kai2△,WU Gang3,Zulpiye Ablikim1,ZHU Qing-feng1,WANG Shao-hai1*,YI Yang-hua4* |
(1. Department of Stomatology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;
2. Laboratory of Marine Biopharmaceutical Research, Food College, Shanghai Ocean University, Shanghai 201306, China;
3. Department of Stomatology, No. 85 Hospital of PLA, Shanghai 200052, China;
4. Research Center for Marine Drugs, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
△Co-first authors
* Corresponding authors) |
| Abstract: |
| Objective To prepare a novel collagen scaffold material using Basa fish (Pangasisus haniltoa) skin as the ingredient and to analyze the structural characteristics, physical properties and degradability of the prepared material, so as to explore whether Basa fish can replace terrestrial mammals for preparing a novel collagen scaffold material. Methods Basa fish skins were lyophilized to obtain the membrane material after repeated degreasing, decolorization and dedoping. Crude protein content was determined by the Kjeldahl method. Structure of the materials and its pore size and distribution were analyzed by scanning electron microscopy (SEM). Porosity was measured by the liquid displacement technique, and tensile strength was tested using universal testing machine. The changes of viscosity with temperatures were detected to determine the denaturation temperature of the material.The material was immersed in the phosphate-buffered solution (0.1 mol/L, pH 7.4), which was placed in a constant temperature shaker at 37℃, and the water absorption and weight loss rates of the material were detected. Results The crude protein content of the collagen scaffold material was 95.2%, with visually uniform thickness. SEM photographs showed that one side of the material had a rough surface and porous structure, on which varying sizes of pores distributed uniformly; the other side was smooth with dense pores. The porosity of the material was (55.50±1.94)%, thickness was (0.66±0.10) mm and tensile strength was (18.82±0.94) MPa. The denaturation temperature of the material was 34℃ before thermo-crosslinking and 36℃ after thermo-crosslinking. The water absorption of the material was (379.77±77.81)% at 48 h. At 28 d after thermo-crosslinking, the degradation rate was (80.22±2.49)%, and the pH value of buffer was 6.67±0.05. Conclusion The collagen scaffold material from Basa fish skin can be made into the biological membrane with uniform thickness, and the membrane comprises double structures: dense layer and loose layer. This material exhibits excellent mechanical strength and appropriate denaturation temperature, but its degradation is fast, which needs further improvement. |
| Key words: Pangasisus haniltoa collagen tissue scaffolds in vitro degradation biodegradable polymer physicochemical properties tissue engineering |
