| 摘要: |
| 目的 制备同时包封IR-780和多柔比星（DOX）的温敏脂质体并进行表征。方法 采用薄膜水化法及硫酸铵梯度法制备载IR-780和DOX温敏脂质体（DOX-IR-780 thermo-sensitive liposome，DITSL）。采用Malvern激光粒度仪检测各脂质体的粒径、表面电位及多分散系数（PDI）；采用激光诱导DITSL升温释药，检测各脂质体的释药特性。结果 IR-780和DOX同时被包封于温敏脂质体，成功制备得DITSL。IR-780和DOX的包封率分别为（94.47±8.57）%、（92.52±7.61）%；平均粒径（138.98±8.74）nm；略带负电位；PDI为0.32±0.02。激光0.8 W/cm2照射5 min，温度最高升到54.2℃，DOX释药率达80.1%。结论 DITSL具有药物包封率较高、粒径大小适宜、光热转化效率高、温度敏感性好的优点，并可通过激光控制释药，为下一步光热-化疗联合治疗肿瘤的研究奠定了基础。 |
| 关键词: 温敏脂质体 多柔比星 IR-780 光热疗法 药物疗法 |
| DOI：10.16781/j.0258-879x.2017.10.1336 |
| 投稿时间：2017-03-18修订日期：2017-06-27 |
| 基金项目:广东省自然科学基金（2016A030313611），广东省科技计划项目（2014A020212255），全军医学科研"十二五"计划课题（CWS12J076）. |
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| Preparation and characterization of thermo-sensitive liposomes loaded with IR-780 and doxorubicin |
| LI Ye-kuo1*,DUAN Wan-lu1,TANG Jia-wei1,HE Jing-ling1,YAN Fei2,ZHENG Hai-rong2 |
(1. Department of Ultrasound, General Hospital of PLA Guangzhou Military Area Command, Guangzhou 510010, Guangdong, China;
2. Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, Guangdong, China
*Corresponding author) |
| Abstract: |
| Objective To prepare and characterize a thermo-sensitive liposomes co-loaded with IR-780 and doxorubicin (DOX). Methods Membrane hydration method and ammonium sulfate gradient method were used to prepare IR-780/DOX thermo-sensitive liposomes (DITSL). The particle size, zeta potential and polydispersity coefficient (PDI) of liposomes were measured by Malvern laser particle size analyzer, and the drug release characteristic of DITSL induced by laser was also detected. Results DITSL loading both IR-780 and DOX was successfully prepared. The encapsulation efficiency of IR-780 and DOX was (94.47±8.57)% and (92.52±7.61)%, respectively; the average particle size was (138.98±8.74) nm, with slight negative potential; the PDI was 0.32±0.02. The drug release rate of DITSL was about 80.1% after laser radiation (0.8 W/cm2, 5 min) and the highest temperature of DITSL was 54.2℃. Conclusion The prepared DITSL has high drug encapsulation efficiency, appropriate particle size, high photo-thermal conversion efficiency, good temperature sensitivity and laser-induced thermal drug release property, which lays a foundation study for the combination treatment of tumors with photo-thermal therapy and chemotherapy. |
| Key words: thermo-sensitive liposomes doxorubicin IR-780 photo-thermal therapy drug therapy |
